Anti-p-Raf-1 Antibody (E-1) recommended for detection of Ser 621 phosphorylated Raf-1 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA

Anti-p-Raf-1 Antibody (E-1): sc-271929

Anti-p-Raf-1 Antibody (E-1) is rated 4.3 out of 5 by 13.
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Datasheets

    • p-Raf-1 Antibody (E-1) is a mouse monoclonal IgG2b κ, cited in 5 publications, provided at 200 µg/ml
    • raised against a short amino acid sequence containing phosphorylated Ser 621 of Raf-1 of human origin
    • p-Raf-1 Antibody (E-1) is recommended for detection of Ser 621 phosphorylated Raf-1 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine and avian
    • Anti-p-Raf-1 Antibody (E-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Raf-1 (E-1): sc-271929.
    • m-IgG2b BP-HRP is the preferred secondary detection reagent for p-Raf-1 Antibody (E-1) for WB and IHC(P) applications. This reagent is now offered in a bundle with p-Raf-1 Antibody (E-1) (see ordering information below).
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What application is the blocking peptide sc-271929 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great blot I obtained some very clear blots from A549 and CL1-5 cell lysates
Date published: 2021-03-17
Rated 4 out of 5 by from good This antibody is not as good as I supposed. We used at a 1:500 dilution.
Date published: 2018-02-14
Rated 5 out of 5 by from Good antibody Good antibody for a very fat target band but have a week band with around 100Kd at healthy human liver sample
Date published: 2017-12-04
Rated 4 out of 5 by from gut für Western Blot Antikörper gutes Signal, wenig Hintergrund Verwendung mit 1:500 Verdünnung
Date published: 2017-10-17
Rated 5 out of 5 by from It works very well. I bought this antibody two month ago. It works very well, especially in background.
Date published: 2017-08-29
Rated 3 out of 5 by from Multiple bands Antibody produced multiple bands at 65 kDa (strongest), 80 kDa and 140 kDa in western blot using 14 ug total protein from human airway smooth muscle cells.
Date published: 2017-08-17
Rated 4 out of 5 by from Works well for both mouse and human lysates in WBs I got a sample of this antibody, I used 1:1000 dilution. Blocking buffer 4%BSA 1%FSG in TBST. I've tried it on mouse mammary gland lysates and it works.
Date published: 2017-08-05
Rated 5 out of 5 by from excellent antibody for westerns Probed at 1/1000X dilution for 1hr and used together with Kappa-binding HRP and the signal was very strong. Excellent antibody.
Date published: 2017-06-21
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