Anti-p-PYK2 Antibody (14F6) recommended for detection of Tyr 402 phosphorylated PYK2 of mouse, rat and human origin by WB and IP

Anti-p-PYK2 Antibody (14F6): sc-81512

Anti-p-PYK2 Antibody (14F6) is rated 3.0 out of 5 by 1.
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Datasheets

    • p-PYK2 Antibody (14F6) is a mouse monoclonal IgG1, cited in 3 publications, provided at 50 µg/0.5 ml
    • raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding tyrosine 402 of PYK2 of human origin
    • recommended for detection of Tyr 402 phosphorylated PYK2 of mouse, rat and human origin by WB and IP; non cross-reactive with the non-phosphorylated form of PYK2 or with unrelated phosphorylation sites
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-PYK2 Antibody (14F6). This work is in progress.
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I am using p-PYK2 (14F6): sc-81512 antibody for immunoprecipitation, how much antibody should I use?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from Worked with transfected lysate by WB with Worked with transfected lysate by WB with some background -SCBT QC
Date published: 2014-04-17
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