Anti-p-PP2A-C alpha / beta Antibody (F-8) recommended for detection of Tyr 307 phosphorylated PP2A-C α and β isoforms of broad species origin by WB, IP, IF, IHC(P) and ELISA

Anti-p-PP2A-Cα/β Antibody (F-8): sc-271903

Anti-p-PP2A-Cα/β Antibody (F-8) is rated 5.0 out of 5 by 8.
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Datasheets

    • p-PP2A-Cα/β Antibody (F-8) is a mouse monoclonal IgG1 κ, cited in 22 publications, provided at 200 µg/ml
    • raised against a short amino acid sequence containing phosphorylated Tyr 307 of PP2A-Cα of mouse origin
    • p-PP2A-C alpha / beta Antibody (F-8) is recommended for detection of Tyr 307 phosphorylated PP2A-C α and β isoforms of broad species origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-p-PP2A-C alpha / beta Antibody (F-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-PP2A-Cα/β (F-8): sc-271903.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for p-PP2A-Cα/β Antibody (F-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with p-PP2A-Cα/β Antibody (F-8) (see ordering information below).
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I have some arabidopsis thaliana samples need to test. Will this antibody work?

Asked by: Anonymous
Thank you for your question. p-PP2A-Cα/β Antibody (F-8) has not yet been tested in arabidopsis thaliana sample and so that is not a recommended species at this time. 感谢您的问题。p-PP2A-Cα/β 抗体 (F-8)还没有检测过拟南芥样本,不推荐检测拟南芥样本。
Answered by: Technical Support 9
Date published: 2020-06-12

Do you have specific technical details about the IHC protocol for this antibody? meaning concentration, kind of antigen retrieval, blocking, etc.

Asked by: AllyValk
Thank you for your question. Please find the link to our IHC protocol below. We recommend starting at a 1:50 primary antibody dilution, sodium citrate with heat at pH 6 for antigen retrieval, and normal serum to block non-specific binding. If you require further assistance, please contact Technical Service. Immunoperoxidase Staining Protocol: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Santa Cruz TS
Date published: 2017-05-19

I have some monkey samples I need to test. Will this antibody work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 4
Date published: 2017-01-13

I would like to use sc-271903 for a double stain. How can I do this when my other antibody is a mouse monoclonal also?

Asked by: Cweed
We recommend using directly conjugated antibodies. This product, sc-271903, is available conjugated to AF488, AF647, and FITC.
Answered by: Technical Support 4
Date published: 2017-01-13
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Rated 5 out of 5 by from Worked well! This antibody worked well with our Rat cell line, REF52.
Date published: 2018-08-14
Rated 5 out of 5 by from Clean and strong signal for p-PP2a (F-8) Received a sample of this antibody and found that it strongly detected p-PP2a in rat primary neuronal cultures at a 1:1000 dilution. I recommend this product for WB application.
Date published: 2018-04-06
Rated 5 out of 5 by from perfect antibody perfect antibody for WB on healthy human liver sample
Date published: 2017-12-04
Rated 5 out of 5 by from pPP2Ac I bought pPP2Ac antibody last month and Its work perfectly.
Date published: 2017-10-31
Rated 5 out of 5 by from Detecting P-PP2A-C We used the antibody (sc-271903) to detect P-PP2A-C in insect samples. The result was quite good.
Date published: 2017-09-11
Rated 5 out of 5 by from Great for IHC This p-PP2A-C alpha/beta antibody shows good results in IHC of human tissue.
Date published: 2017-01-14
Rated 5 out of 5 by from wonderful Antibody for p-pp2a got the free sample for this P-PP2A one month ago. Tested it. Worked perfect. Placed one full packge order soon.
Date published: 2016-11-11
Rated 5 out of 5 by from Signal via mmunoperoxidase staining of formalin Signal via mmunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear staining of subset of glandular cells. -SCBT QC
Date published: 2013-07-18
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