p-Pol II Antibody (8A7)detects Ser 1801 phosphorylated RNA polymerase II of human origin and correspondingly phosphorylated RNA polymerase II of mouse, rat and S. cerevisiae origin by WB, IP, IF and IHC(P)
p-Pol II Antibody (8A7) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
raised against a synthetic peptide containing phosphorylated Ser 1801 of p-Pol II of human origin
recommended for detection of Ser 1801 phosphorylated RNA polymerase II of human origin and correspondingly phosphorylated RNA polymerase II of mouse, rat and S. cerevisiae origin by WB, IP, IF and IHC(P)
TransCruz reagent for Gel Supershift and ChIP applications (sc-13583 X, 200 µg/0.1 ml)
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
When should the TransCruz reagent sc-13583 X be used instead of the standard form sc-13583?
Asked by: Trav11
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
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