p-Pol II Antibody (8A7)detects Ser 1801 phosphorylated RNA polymerase II of human origin and correspondingly phosphorylated RNA polymerase II of mouse, rat and S. cerevisiae origin by WB, IP, IF and IHC(P)

p-Pol II Antibody (8A7): sc-13583

p-Pol II Antibody (8A7) is rated 4.9 out of 5 by 7.
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Datasheets

    • p-Pol II Antibody (8A7) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • raised against a synthetic peptide containing phosphorylated Ser 1801 of p-Pol II of human origin
    • recommended for detection of Ser 1801 phosphorylated RNA polymerase II of human origin and correspondingly phosphorylated RNA polymerase II of mouse, rat and S. cerevisiae origin by WB, IP, IF and IHC(P)
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-13583 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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When should the TransCruz reagent sc-13583 X be used instead of the standard form sc-13583?

Asked by: Trav11
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from normal use During normal use, the traits in WB are good. The mutation state in the reference object is not obvious
Date published: 2017-01-18
Rated 4 out of 5 by from Pretty good antibody Pretty good antibody. We have used a different lot in the past and it worked really well. No big complaints.
Date published: 2015-06-19
Rated 5 out of 5 by from Use it routinely Use it routinely, works well in WB, no complaints
Date published: 2015-04-21
Rated 5 out of 5 by from Strong nuclear localization in IF in methanol Strong nuclear localization in IF in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Worked well in IF in monkey CV Worked well in IF in monkey CV-1 cells. -SCBT Publication Review
Date published: 2015-03-05
Rated 5 out of 5 by from Good antibody for ChIP in human HEK293 Good antibody for ChIP in human HEK293, MCF-7 cells. -SCBT Publication Review
Date published: 2015-01-14
Rated 5 out of 5 by from Strong band in WB in HeLa whole cell lysates Strong band in WB in HeLa whole cell lysates. -SCBT QC
Date published: 2013-08-20
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