Anti-p-PLC gamma 1 Antibody (pY783.27) recommended for detection of Tyr 783 phosphorylated PLC γ1 of mouse, rat and human origin by WB and IP

Anti-p-PLC γ1 Antibody (pY783.27): sc-136186

Anti-p-PLC γ1 Antibody (pY783.27) is rated 3.5 out of 5 by 6.
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Datasheets

    • p-PLC γ1 Antibody (pY783.27) is a mouse monoclonal IgG1 κ, cited in 4 publications, provided at 200 µg/ml
    • raised against a short amino acid sequence containing phosphorylated Tyr 783 of PLC γ1 of human origin
    • recommended for detection of Tyr 783 phosphorylated PLC γ1 of mouse, rat and human origin by WB and IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-PLC γ1 (pY783.27): sc-136186.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-PLC γ1 Antibody (pY783.27) for WB applications. These reagents are now offered in bundles with p-PLC γ1 Antibody (pY783.27) (see ordering information below).
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I am using p-PLC γ1 (pY783.27): sc-136186 antibody for immunoprecipitation, how much antibody should I use?

Asked by: cjMara
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Got bands! Got clear bands and no confusing non-specific binding
Date published: 2019-03-04
Rated 1 out of 5 by from Sorry, it did not work It did not detect any signal of p-PLC γ1 in both human and mouse samples.
Date published: 2018-05-23
Rated 5 out of 5 by from excellent It worked very well for samples from hela cells. Samples from mouse osteoclasts should be concentrated.
Date published: 2018-04-05
Rated 4 out of 5 by from Works well! Works well for my application... where probing mouse lysates at 1:200 dilution via WB.
Date published: 2018-03-23
Rated 3 out of 5 by from No protein bands I used the same samples to check this product and sc-7290. The result of sc-7290 was good, while I didn't get good result.
Date published: 2018-01-15
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