Anti-p-p21 Waf1/Cip1/CDKN1A Antibody (D-4) recommended for detection of Ser 146 phosphorylated p21 Waf1/Cip1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-p-p21 Waf1/Cip1/CDKN1A Antibody (D-4): sc-377515

Anti-p-p21 Waf1/Cip1/CDKN1A Antibody (D-4) is rated 3.2 out of 5 by 5.
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Datasheets

    • p-p21 Waf1/Cip1/CDKN1A Antibody (D-4) is a mouse monoclonal IgG3 κ, cited in 4 publications, provided at 200 µg/ml
    • recommended for detection of Ser 146 phosphorylated p21 Waf1/Cip1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, bovine and porcine
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-p21 Waf1/Cip1/CDKN1A (D-4): sc-377515.
    • m-IgG3 BP-HRP is the preferred secondary detection reagent for p-p21 Waf1/Cip1/CDKN1A Antibody (D-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with p-p21 Waf1/Cip1/CDKN1A Antibody (D-4) (see ordering information below).
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What application is the blocking peptide sc-377515 P appropriate for?

Asked by: Trav11
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from No bands in WB I used it in western blot (15 ug protein loaded per lane) and even at a dilution of 1:100 at I got no signal, whereas I received a robust signal with Anti-Cip1 antibody from BD
Date published: 2017-10-18
Rated 4 out of 5 by from Good for WB 1:200 diluted for wb of rat protein ,the band is special and strong.
Date published: 2017-08-08
Rated 3 out of 5 by from 用着一般 抗体用着一般,用的结直肠癌细胞DLD-1,还用了HEK293T细胞,上样量达到120ug才看到有条带,效价也不高1:300用的。
Date published: 2017-07-12
Rated 4 out of 5 by from Works on mouse S-146-p21 in IB This antibody is sensitive to milk/Blotto. Requires 75ug of mouse cellular protein per lane to detect endogenous levels of P-S146-p21 and must be transferred to nitrocellulose. Works at 1/500 dilution in BSA/NGS/PBST buffer.
Date published: 2016-11-03
Rated 4 out of 5 by from Good antibody for Western with human recombinant Good antibody for Western with human recombinant p21 fusion proteins. -SCBT QC
Date published: 2015-03-03
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