p-NOS3 Antibody (pS1177.39)recommended for detection of Ser 1177 phosphorylated NOS3 of human origin by WB, IP, IF and IHC(P)

p-NOS3 Antibody (pS1177.39): sc-293032

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Datasheets
    • p-NOS3 Antibody (pS1177.39) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a short amino acid sequence containing phosphorylated Ser 1177 of NOS3 of human origin
    • recommended for detection of Ser 1177 phosphorylated NOS3 of human origin by WB, IP, IF and IHC(P)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Can p-NOS3 (pS1177.39): sc-293032 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, p-NOS3 (pS1177.39): sc-293032 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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