p-NOS3 Antibody (15E2)recommended for detection of Ser 1177 phosphorylated NOS3 of mouse, rat and human origin by WB and IP

p-NOS3 Antibody (15E2): sc-81510

p-NOS3 Antibody (15E2) is rated 4.2 out of 5 by 5.
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Datasheets
    • p-NOS3 Antibody (15E2) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
    • raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding serine 1177 of NOS3 of human origin
    • recommended for detection of Ser 1177 phosphorylated NOS3 of mouse, rat and human origin by WB and IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-NOS3 (15E2): sc-81510.
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I am using p-NOS3 (15E2): sc-81510 antibody for immunoprecipitation, how much antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from phospho eNOS in keratinocytes Good p-eNOS signal in keratinocytes by WB and fibroblasts
Date published: 2018-11-16
Rated 4 out of 5 by from weak band I use 1:200 in WB,the band is very weak,the antibody for p-nos3 is difficult to use of various of bands of antibody such as CST and abcam,so I forgive it.
Date published: 2018-03-09
Rated 4 out of 5 by from Quality band in endothelial cells Got a good band in HMvECs for Western blot. Would recommend
Date published: 2018-02-19
Rated 4 out of 5 by from A little weak 1:500 diluted with 1 %BSA for wb of rat protein,the band is little weak.
Date published: 2017-07-02
Rated 4 out of 5 by from Good specificity and signal strength in Good specificity and signal strength in untreated (A) and pervanadate-treated (B) bend 3 whole cell lysates. -SCBT QC
Date published: 2013-06-11
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