Anti-p-nestin Antibody (A-4) recommended for detection of Thr 316 phosphorylated Nestin of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-p-nestin Antibody (A-4): sc-377538

Anti-p-nestin Antibody (A-4) is rated 5.0 out of 5 by 1.
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Datasheets

    • p-nestin Antibody (A-4) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • recommended for detection of Thr 316 phosphorylated Nestin of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and bovine and porcine
    • See nestin (Rat-401): sc-33677 for p-nestin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-nestin (A-4): sc-377538.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-nestin Antibody (A-4). This work is in progress.
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What application is the blocking peptide sc-377538 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Satisfactory Western blot data of nestin Satisfactory Western blot data of nestin phosphorylation in untreated and lambda protein phosphatase treated SH-SY5Y whole cell lysates. -SCBT QC
Date published: 2014-06-21
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