p-MYPT1 Antibody (F-11)recommended for detection of Ser 695 phosphorylated MYPT1 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA

p-MYPT1 Antibody (F-11): sc-377531

p-MYPT1 Antibody (F-11) is rated 5.0 out of 5 by 4.
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Datasheets

    • p-MYPT1 Antibody (F-11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • p-MYPT1 Antibody (F-11) is recommended for detection of Ser 695 phosphorylated MYPT1 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA
    • p-MYPT1 Antibody (F-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-377531 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-MYPT1 (F-11): sc-377531.
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What application is the blocking peptide sc-377531 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from IHC of rat adipocyte tissue IHC of rat adipocyte tissue result seems to be reliable
Date published: 2017-11-19
Rated 5 out of 5 by from Very specific, excellent antibody Whoever works with pMYPT1 (Thr850) antibody from whichever company, has to know that it is very tricky, either it shows the right bands but not the state, or it is shifted, usually bellow, and it shows then what it is suppose to show. This Santa Cruz new monoclonal antibody is very specific and it shows good bands at ~75kDa (the size as with Millipore and other companies pMYPT1) in (1:500) in rat lungs, heart and brain samples.
Date published: 2016-11-02
Rated 5 out of 5 by from WB positive using HCT WB positive using HCT-8 whole cell lysates. -SCBT QC
Date published: 2015-05-10
Rated 5 out of 5 by from Worked great in Western with treated and Worked great in Western with treated and untreated HeLa whole cell lysates. -SCBT QC
Date published: 2014-09-03
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