Anti-p-MEK-1 Antibody (47.Ser 222) recommended for detection of Ser 222 phosphorylated MEK-1 and Ser 222 phosphorylated MEK-2 of mouse, rat and human origin by WB, IP and ELISA

Anti-p-MEK-1 Antibody (47.Ser 222): sc-136542

Anti-p-MEK-1 Antibody (47.Ser 222) is rated 3.2 out of 5 by 5.
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Datasheets

    • p-MEK-1 Antibody (47.Ser 222) is a mouse monoclonal IgA, cited in 4 publications, provided at 200 µg/ml
    • raised against a short amino acid sequence containing phosphorylated Ser 222 of MEK-1 of human origin
    • recommended for detection of Ser 222 phosphorylated MEK-1 and Ser 222 phosphorylated MEK-2 of mouse, rat and human origin by WB, IP and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-MEK-1 (47.Ser 222): sc-136542.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-MEK-1 Antibody (47.Ser 222). This work is in progress.
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What is the recommended starting dilution for Western blot for p-MEK-1 (47.Ser 222): sc-136542 monoclonal antibody?

Asked by: jerojero
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-136542.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from Not good I tested it in our Hela cell lysate, and it did not work at all
Date published: 2018-04-03
Rated 4 out of 5 by from Working good! I got this for the free sample trial, and treated on B16 cells. It worked well.
Date published: 2018-03-07
Rated 1 out of 5 by from Didnot work for WB did not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from Publishable Western blot results using human Publishable Western blot results using human osteosarcoma cell lines (U2OS and HOS). -SCBT Publication Review
Date published: 2015-01-23
Rated 5 out of 5 by from Good WB publishable data gathered with human Good WB publishable data gathered with human primary osteoblasts and Synovial fibroblasts. -SCBT Publication Review
Date published: 2014-05-24
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