Anti-p-MEK-1/2 Antibody (7E10) recommended for detection of Ser 218 and 222 phosphorylated MEK-1 and Ser 222 and 226 phosphorylated MEK-2 of mouse, rat, human and canine origin by WB and IP

Anti-p-MEK-1/2 Antibody (7E10): sc-81503

Anti-p-MEK-1/2 Antibody (7E10) is rated 4.0 out of 5 by 5.
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Datasheets

    • p-MEK-1/2 Antibody (7E10) is a mouse monoclonal IgG1, cited in 30 publications, provided at 50 µg/0.5 ml
    • raised against phosphopeptide corresponding to amino acid residues surrounding the T-E-Y motif of MEK-1/2 of human origin
    • recommended for detection of Ser 218 and 222 phosphorylated MEK-1 and Ser 222 and 226 phosphorylated MEK-2 of mouse, rat, human and canine origin by WB and IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-MEK-1/2 (7E10): sc-81503.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-MEK-1/2 Antibody (7E10) for WB applications. These reagents are now offered in bundles with p-MEK-1/2 Antibody (7E10) (see ordering information below).
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Hi I need an option for IHC(P) for p-MEK-1/2 Antibody (7E10): sc-81503, any suggestions

Asked by: Parajuli
Thank your for your question. This antibody, sc-81503, is not recommended or warrantied for IHC(P). We do not currently offer a p-MEK-1/2 antibody recommended for this use. However, if you want to try it, you can find our protocol here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Tech Service
Date published: 2019-02-18

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-02-17
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Rated 3 out of 5 by from Not very intense bands The blot was quite unclear and the bands not very intense. I used A549 whole cell lysates and 60μg total protein. The antibody was incubated in milk 3% v/w
Date published: 2021-03-17
Rated 5 out of 5 by from Excellent! This antibody is recommended for detection of Ser 218 and 222 phosphorylated MEK-1 and Ser 222 and 226 phosphorylated MEK-2 of mouse, human and canine origin by WB and IP
Date published: 2017-01-20
Rated 4 out of 5 by from Light Light, positive band in WB in untreated and PDGF treated NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-03-06
Rated 4 out of 5 by from Good Good, clear band in WB in serum-starved, PMA-treated HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-03
Rated 4 out of 5 by from Positive band seen in WB in EGF stimulated Positive band seen in WB in EGF stimulated and pervanadate treated A549 whole cell lysate. -SCBT QC
Date published: 2014-07-28
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