Anti-p-Lck Antibody (pY505.4) recommended for detection of Tyr 505 phosphorylated Lck of mouse, rat and human origin by WB and IP

Anti-p-Lck Antibody (pY505.4): sc-136184

Anti-p-Lck Antibody (pY505.4) is rated 4.3 out of 5 by 4.
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Datasheets

    • p-Lck Antibody (pY505.4) is a mouse monoclonal IgG1 κ, cited in 1 publications, provided at 200 µg/ml
    • raised against a short amino acid sequence containing phosphorylated Tyr 505 of Lck of human origin
    • recommended for detection of Tyr 505 phosphorylated Lck of mouse, rat and human origin by WB and IP
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for p-Lck Antibody (pY505.4) for WB applications. This reagent is now offered in a bundle with p-Lck Antibody (pY505.4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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I am using p-Lck (pY505.4): sc-136184 antibody for immunoprecipitation, how much antibody should I use?

Asked by: jenniferc
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Great monoclonal for p-Lck This antibody gives beautiful westerns, nice specific single bands in treated cells. Great choice for any studies on phosphorylated Lck.
Date published: 2017-02-06
Rated 4 out of 5 by from Western blot analysis of Lck phosphorylation Western blot analysis of Lck phosphorylation in untreated, pervanadate treated & pervanadate treated and lambda protein phosphatase treated Jurkat whole cell lysates. -SCBT QC
Date published: 2015-06-25
Rated 4 out of 5 by from Good specificity and signal strength via Good specificity and signal strength via western blotting with transfected lysates. -SCBT QC
Date published: 2015-02-05
Rated 4 out of 5 by from Clean band in Western blot analysis of Lck Clean band in Western blot analysis of Lck expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2014-06-27
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