Anti-p-JNK Antibody (G-7) recommended for detection of Thr 183 and Tyr 185 phosphorylated JNK1, JNK2 and JNK3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Anti-p-JNK Antibody (G-7): sc-6254

Anti-p-JNK Antibody (G-7) is rated 4.7 out of 5 by 68.
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Datasheets

    • p-JNK Antibody (G-7) is a mouse monoclonal IgG1 κ, cited in 1,280 publications, provided at 200 µg/ml
    • Anti-p-JNK Antibody (G-7) is recommended for detection of Thr 183 and Tyr 185 phosphorylated JNK1, JNK2 and JNK3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-p-JNK Antibody (G-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA; and to either TRITC or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-JNK (G-7): sc-6254.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-JNK Antibody (G-7) for WB and IHC(P) applications. These reagents are now offered in bundles with p-JNK Antibody (G-7) (see ordering information below).
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Hello, can i use this Antibody to detect phosphorylation in primary human fibroblasts?

Asked by: Alexander M
Thank you for your question. p-JNK Antibody (G-7): sc-6254 detects Thr 183 and Tyr 185 phosphorylated JNK1, JNK2 and JNK3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. It is only specific against these phosphorylated residues and does not detect JNK that does not possess these phosphorylations.
Answered by: Tech Support Europe
Date published: 2019-08-29

Hi. Hope you are well. I am planning to use p-JNK antibody in western blotting in mice brain areas of hippocampus, cortex and nucleus accumbens. Is this product suitable for it as I cannot tell from the references. Thanks

Asked by: Lalji
Thank you for your question. Yes, this antibody is recommended for Western blotting in samples of mouse origin and is specific for phosphorylated JNK. There are product citations specifically in mouse brain. Please contact our Technical Service Department if you would like us to provide specific product citations as a reference.
Answered by: Technical Support
Date published: 2019-05-07

can I use sc-6254 PE in mouse cells for flow cytometry. ....any publication data?

Asked by: Thom
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-01-26

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-14

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Cweed
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. SC-6254 is currently available conjugated to AF488, AF647, and FITC.
Answered by: TechService7
Date published: 2016-12-14
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Rated 5 out of 5 by from great antibody! This primary antibody is useful in western blotting
Date published: 2021-02-08
Rated 5 out of 5 by from Sc-6254 - p-JNK Antibody Review The p-JNK Antibody (Sc-6254) was used for Western Blot analysis and Immunohistochemistry analysis and gives excellent data in the same.
Date published: 2020-10-20
Rated 4 out of 5 by from Good for WB This antibody is high specific and high sensitivity in human liver cell lines. Highly recommend antibody
Date published: 2019-11-02
Rated 4 out of 5 by from excellent This antibody is high specific and high sensitivity in human liver cell lines. Highly recommend this antibody
Date published: 2019-10-23
Rated 5 out of 5 by from Great images Stained PFA-fixed human lung cancer cells by immunofluorescence.
Date published: 2019-06-26
Rated 2 out of 5 by from Weak, non-specific Western in rat INS-1 cells 60 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a weak, specific, anisomycin responsive band at 54 kD and a strong, non-responsive, non-specific band ~46 kD. Lack of specificity confirmed using another p-JNK antibody. The attached picture has 30 and 90 ug loads -/+ anisomycin
Date published: 2019-05-22
Rated 5 out of 5 by from Works great in IF This antibody works well in immunofluorescence assay on human lung cancer cells
Date published: 2019-05-14
Rated 5 out of 5 by from Great specific antibody Clear and specific two bands for BV-2 and N9 microglial cell extracts, varying accordingly to the stimuli (1:200 in 5% non-fat milk 1X TBS, overnight).
Date published: 2018-11-09
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