Anti-p-JNK Antibody (9H8) recommended for detection of both Tyr 185 phosphorylated JNK1, JNK2 and JNK3 and Thr 183 and Tyr 185 dually phosphorylated JNK1, JNK2 and JNK3 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-p-JNK Antibody (9H8): sc-81502

Anti-p-JNK Antibody (9H8) is rated 4.8 out of 5 by 4.
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Datasheets

    • p-JNK Antibody (9H8) is a mouse monoclonal IgG1, cited in 34 publications, provided at 50 µg/0.5 ml
    • raised against phosphopeptide corresponding to amino acid residues surrounding the pThr-Gly/Pro-pTyr motif of JNK of human origin
    • recommended for detection of both Tyr 185 phosphorylated JNK1, JNK2 and JNK3 and Thr 183 and Tyr 185 dually phosphorylated JNK1, JNK2 and JNK3 of mouse, rat and human origin by WB, IP, IF and ELISA; non cross-reactive with the non-phosphorylated form of JNK
    • See p-JNK (G-7): sc-6254 for p-JNK antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-JNK Antibody (9H8). This work is in progress.
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I am using p-JNK (9H8): sc-81502 for immunofluorescence, what dilution of this antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from This antibody worked well in detecting p This antibody worked well in detecting p-JNK in western blotting assay.
Date published: 2016-09-02
Rated 5 out of 5 by from WB: Positive in mouse L929 cells WB: Positive in mouse L929 cells. -SCBT Publication Review
Date published: 2015-03-12
Rated 5 out of 5 by from Publication in IP and WB with human HeLa samples Publication in IP and WB with human HeLa samples. -SCBT Publication Review
Date published: 2015-03-10
Rated 5 out of 5 by from Worked well for Western Worked well for Western, publication in A549 cells. -SCBT Publication Review
Date published: 2014-02-17
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