p-HSP 27 Antibody (5B9)recommended for detection of Ser 82 phosphorylated HSP 27 of mouse, rat, human and canine origin by WB and IP

p-HSP 27 Antibody (5B9): sc-81498

p-HSP 27 Antibody (5B9) is rated 4.0 out of 5 by 2.
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Datasheets
    • p-HSP 27 Antibody (5B9) is a mouse monoclonal IgG2a provided at 50 µg/0.5 ml
    • raised against phosphopeptide corresponding to amino acid residues surrounding serine 82 of HSP 27 of human origin
    • recommended for detection of Ser 82 phosphorylated HSP 27 of mouse, rat, human and canine origin by WB and IP; non cross-reactive with non-phosphorylated HSP 27 or unrelated p-Ser proteins
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I am using p-HSP 27 (5B9): sc-81498 antibody for immunoprecipitation, how much antibody should I use?

Asked by: jerojero
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Reduction in signal supporting specificity Reduction in signal supporting specificity via western blot analysis of HSP 27 phosphorylation in serum starved Hep G2 cells and serum starved Hep G2 cells treated with 10 ng/ml EGF for 5 min, 15 min, 30 min, 1 hr, 2 hrs, 4 hrs and 8 hrs. -SCBT QC
Date published: 2015-03-22
Rated 4 out of 5 by from Supporting western blot analysis of HSP 27 Supporting western blot analysis of HSP 27 phosphorylation in non-transfected 293T, mouse HSP 27 transfected 293T and ECV304 whole cell lysates. -SCBT QC
Date published: 2015-01-25
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