Anti-p-GSK-3 beta Antibody (F-2) recommended for detection of Ser 9 phosphorylated GSK-3β of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA

Anti-p-GSK-3β Antibody (F-2): sc-373800

Anti-p-GSK-3β Antibody (F-2) is rated 4.7 out of 5 by 28.
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Datasheets

    • p-GSK-3β Antibody (F-2) is a mouse monoclonal IgG1 κ, cited in 54 publications, provided at 200 µg/ml
    • p-GSK-3 beta Antibody (F-2) is recommended for detection of Ser 9 phosphorylated GSK-3β of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • Anti-p-GSK-3 beta Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-GSK-3β (F-2): sc-373800.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-GSK-3β Antibody (F-2). This work is in progress.
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Is this antibody validated for decalcified paraffin embedded mouse femur ? 

Asked by: Marianne200
Thank you for your question. This antibody is recommended for use in formalin-fixed paraffin-embedded sections, or for IHC(P), of mouse origin. We have not specifically tested it in femur.
Answered by: Technical Support
Date published: 2019-07-13

Is this antibody specific for the phosphorylated protein, and will not cross-react with the non-phosphorylated form of the protein?

Asked by: cjMara
Yes. p-GSK-3β (F-2): sc-373800 monoclonal antibody is specific for GSK-3β phosphorylated at Ser 9. The antibody has been validated through a series Western blot experiments using lambda phosphatase treated lysates to demonstrate phospho-specificity.
Answered by: Technical Support 7
Date published: 2017-01-25
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Rated 5 out of 5 by from 美しいバンドが検出されます。 WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 5 out of 5 by from Excellent by IF This antibody works great for immunofluorescence to detect phosph-GSK3beta signalling events.
Date published: 2019-05-14
Rated 3 out of 5 by from decent, but non-specific bands Decent antibody. used at 1:2000 on 15ug of bone homogenates from mice femurs.
Date published: 2018-05-22
Rated 5 out of 5 by from It works well I'm giving my review on the sample of this product. I got a good result.
Date published: 2018-05-06
Rated 5 out of 5 by from Ottimo funzionamento in Western-blot Ho testato questo anticorpo su cellule HeLa un mese fa. Funziona bene: Diluzione 1:1000 su 20 ug di total extracts.
Date published: 2017-11-07
Rated 5 out of 5 by from western结果很好 用来做western条带大小和检测结果都很好,非常满意。 另外,抗体趋势也很强,浓度推荐1:500,用于人小管上皮细胞检验
Date published: 2017-10-13
Rated 5 out of 5 by from Very good I had tested it in C42B cell lines. It works very good.
Date published: 2017-10-11
Rated 5 out of 5 by from Great antibody A clear band can be detected at 47KD in WB at 1:1000 dilution.
Date published: 2017-10-10
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