Anti-p-GSK-3 alpha Antibody (9B8) recommended for detection of Ser 21 phosphorylated GSK-3α of mouse, rat, human and canine origin by WB, IP and IF

Anti-p-GSK-3α Antibody (9B8): sc-81497

Anti-p-GSK-3α Antibody (9B8) is rated 4.0 out of 5 by 3.
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Datasheets

    • p-GSK-3α Antibody (9B8) is a mouse monoclonal IgG1, cited in 2 publications, provided at 50 µg/0.5 ml
    • raised against phosphopeptide corresponding to amino acid residues surrounding serine 21 of GSK-3α of human origin
    • recommended for detection of Ser 21 phosphorylated GSK-3α of mouse, rat, human and canine origin by WB, IP and IF; non cross-reactive with the homologous phosphorylation site in GSK-3β
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-GSK-3α Antibody (9B8). This work is in progress.
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I am using p-GSK-3α (9B8): sc-81497 for immunofluorescence, what dilution of this antibody should I use?

Asked by: TinTin
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Clear Clear, specific band in WB in untreated human GSK-3 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-01-18
Rated 4 out of 5 by from Strong positive band for WB in human GSK Strong positive band for WB in human GSK-3 transfected 293T and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-01-11
Rated 4 out of 5 by from Robust signal in WB in human GSK Robust signal in WB in human GSK-3 transfected 293T whole cell lysate. -SCBT QC
Date published: 2014-08-30
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