Anti-p-FAK Antibody (2D11) recommended for detection of Tyr 397 phosphorylated FAK of mouse, rat, human and canine origin by WB and IP

Anti-p-FAK Antibody (2D11): sc-81493

Anti-p-FAK Antibody (2D11) is rated 4.1 out of 5 by 23.
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Datasheets

    • p-FAK Antibody (2D11) is a mouse monoclonal IgG1 κ, cited in 47 publications, provided at 50 µg/0.5 ml
    • raised against phosphopeptide corresponding to amino acid residues surrounding tyrosine 397 of FAK of human origin
    • recommended for detection of Tyr 397 phosphorylated FAK of mouse, rat, human and canine origin by WB and IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-FAK (2D11): sc-81493.
    • m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-FAK Antibody (2D11) for WB applications. These reagents are now offered in bundles with p-FAK Antibody (2D11) (see ordering information below).
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What is the best secondary antibody for this mouse monoclonal?

Asked by: Anna99
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-13

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with p-FAK Antibody (2D11): sc-81493 ?

Asked by: Anna99
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-13
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Rated 4 out of 5 by from Works well in WB This antibody works well in WB, I used it to detect p-FAK in Huh7, dil:1:500
Date published: 2020-02-21
Rated 1 out of 5 by from Not working antibody I recived a free sample from this antibody and it din´t work. I am working with MDCK cells also I charge a sample from HeLa as a positive control, I used 1:50, 1:200 and 1:500 dilution and in any of those I saw a band on the western blot. I used GAPDH as a charge control and I tested my secondary antibody as well with no failures.
Date published: 2019-07-22
Rated 2 out of 5 by from Non-specific bands obscure results I tried this on lysates from both mouse and humans cells in experiments where I was blotting for several other proteins at the same time. The others worked, but there was a strong band too large to be P-FAK that made it very difficult to get a result. The FAK (B-8) antibody worked to confirm that this larger band was not FAK. I suppose you could get a result if you ran a low % gel for a long time to get more separation.
Date published: 2019-01-18
Rated 5 out of 5 by from good band in WB Works good in WB with HCECs human corneal epithelial cells .
Date published: 2018-11-22
Rated 5 out of 5 by from Nice band in WB p-FAK had strong band in WB in human leukemia cell line Molt4
Date published: 2018-07-09
Rated 5 out of 5 by from Good for WB in cell lysates WB using p-FAK (2D11) 1:1000 in 76NE6 cells, clear band detected.
Date published: 2018-05-21
Rated 5 out of 5 by from Fantastic for Western Blot Used this antibody at 1:1000 dilution on a western blot with 100 ug of protein from tissue and another blot with 60 ug of protein from cancer cell lines. Both blots came out absolutely crystal clear.
Date published: 2018-02-21
Rated 5 out of 5 by from Good antibody! 1:500 diluted for wb of rat protein,the band is quite strong and special!
Date published: 2017-12-30
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