Anti-p-ERK Antibody (E-4) recommended for detection of ERK 1 phosphorylated at Tyr 204 and correspondingly phosphorylated ERK 2 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA

Anti-p-ERK Antibody (E-4): sc-7383

Anti-p-ERK Antibody (E-4) is rated 4.7 out of 5 by 95.
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Datasheets

    • p-ERK Antibody (E-4) is a mouse monoclonal IgG2a κ, cited in 3,018 publications, provided at 200 µg/ml
    • p-ERK Antibody (E-4) is recommended for detection of ERK 1 phosphorylated at Tyr 204 and correspondingly phosphorylated ERK 2 of mouse, rat, human and origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • Anti-p-ERK Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA; and to either TRITC, PerCP or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-ERK (E-4): sc-7383.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for p-ERK Antibody (E-4) for WB and IHC(P) applications. These reagents are now offered in bundles with p-ERK Antibody (E-4) (see ordering information below).
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I am writing to ask if your Alexafluor-labeled p-ERK antibody, cat# sc-7383 produces a good immunofluorescent signal, and do you have any images you'd be willing to share ? I'd like to see the staining pattern before I purchase it...many thanks !!!!

Asked by: Anonymous
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Tech Service 1
Date published: 2021-10-15

Hello, I work in lab at UCSC and was wondering if I could obtain a sample of this p-ERK antibody (sc-7383)? Thank you, Celine

Asked by: cneudorf
Thank you for your question. Please contact our Technical Service Department directly to arrange a free 10 µg sample of p-ERK (E-4): sc-7383.
Answered by: Tech Support Europe
Date published: 2021-01-08

Hello Sir/Mam Should we have to use 5% BSA as the blocking agent, diluent for primary antibody and secondary antibody or is 5% milk okay to use.

Asked by: Neha attal
Thank you for your question. We recommend using our plant protein-based UltraCruz® Blocking Reagent: sc-516214 for blocking purposes. BSA- or milk powder-based may also be used instead: If signal strength is too low, BSA may be the better choice, whereas a milk powder blocking buffer is better suited to block promiscuous, unspecific signals.
Answered by: Tech Support Europe
Date published: 2020-06-30

Does sc-7383 recognize dually phosphorylated ERK1/2? What is the biological role of mono-phosphorylated ERK1/2 and does it necessarily undergo threonine phosphorylation subsequently? Thank you.

Asked by: Biologist
Thank you for the question. p-ERK Antibody (E-4): sc-7383 is recommended for detection of ERK 1 phosphorylated Tyr at the position 204 with a mw of ~44kDa. Correspondingly phosphorylated ERK 2 will also be detected at ~42kDa. It was not examined if the antibody has a different binding capacity of the dually phosphorylated vs. the mono phosphorylated ERK proteins. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Regarding additional pathway information I suggest checking the protein info https://www.ncbi.nlm.nih.gov/protein/P27361 and the available product citations and background references listed for this product.
Answered by: Technical Support Europe
Date published: 2018-02-01

Hi, Officer: May I ask a FREE 10 µg sample of p-ERK (E-4): sc-7383 for my Experiment ? Thanks!!!!!!!!!!

Asked by: Ling Sun
Thank you for your question. Yes, for a limited time we are offering a free 10 µg sample of our mouse monoclonal antibody, p-ERK (E-4): sc-7383. Please contact our Technical Service Department to request a free sample. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website
Answered by: Technical Support
Date published: 2017-06-12

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Anonymous
Yes. We recommend using a directly conjugated version of p-ERK Antibody (E-4) sc-7383. This antibody is currently available conjugated to AlexaFluor 405 (sc-7383 AF405), AlexaFluor 488 (sc-7383 AF488), AlexaFluor 647 (sc-7383 AF647), biotin (sc-7383 B), fluorescein (sc-7383 FITC), HRP (sc-7383 HRP), PCPC5 (sc-7383 PCPC5), phycoerythrin (sc-7383 PE), PerCP (sc-7383 PerCP), and TRITC (sc-7383 TRITC).
Answered by: Tech Service 6
Date published: 2016-12-14

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Anonymous
We recommend using one our Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 6
Date published: 2016-12-14

What is the amino acid sequence of the blocking peptide (sc-7383 P)?

Asked by: tarotaro27
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Rated 5 out of 5 by from Accurate and reproducible This product is excellent, just as the ones I previously bought.
Date published: 2021-08-27
Rated 5 out of 5 by from Great features! his primary antibody was able to detect phosphorylated ERK (p42 and p44) giving well defined bands.
Date published: 2021-07-08
Rated 5 out of 5 by from pERK This primary antibody was able to detect phosphorylated ERK (p42 and p44) giving well defined bands.
Date published: 2021-05-17
Rated 5 out of 5 by from Nice antibody for pERK Very reliable signal in mouse skeletal muscle. Strong signal at 1:500 dilution and low background signal.
Date published: 2021-03-19
Rated 5 out of 5 by from excellent blot I detected the p-ERK in lysates from A549 lung adenocarcinoma cells.
Date published: 2021-03-17
Rated 5 out of 5 by from Great antibodies We have been using this antibody in both human cell lines and Xenopus egg extracts to detect activated ERK. In both cases, this antibody has always given clear cut results, and we highly recommend this antibody.
Date published: 2021-02-27
Rated 4 out of 5 by from ストレス応答の検出に適している 1ヶ月前にサンプルを試してみました。放射線照射サンプルを用いてWBを行ったところ、照射によるERKのリン酸化を検出することができた。
Date published: 2020-03-13
Rated 4 out of 5 by from excellent This antibody is high specific and high sensitivity in human cell lines. Highly recommend this antibody
Date published: 2019-10-23
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