Anti-p-ERK 1/2 Antibody (pT202/pY204.22A)recommended for detection of Thr 202 and Tyr 204 dually phosphorylated ERK 1 of human origin, correspondingly dually phosphorylated ERK 1 of mouse and rat origin; and...by WB, IF and IHC(P)
p-ERK 1/2 Antibody (pT202/pY204.22A) is a mouse monoclonal IgG1 κ, cited in 96 publications, provided at 200 µg/ml
raised against a short amino acid sequence containing dually phosphorylated Thr 202 and Tyr 204 of ERK 1 of rat origin
recommended for detection of Thr 202 and Tyr 204 dually phosphorylated ERK 1 of human origin, correspondingly Thr 203 and Tyr 205 dually phosphorylated ERK 1 of mouse and rat origin; and Thr 185 and Tyr 187 dually phosphorylated ERK 2 of human origin, correspondingly Thr 183 and Tyr 185 dually phosphorylated ERK 2 of mouse and rat origin by WB, IF and IHC(P)
available conjugated to agarose for IP; and to HRP for WB, IHC(P) and ELISA
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-ERK 1/2 (pT202/pY204.22A): sc-136521.
At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-ERK 1/2 Antibody (pT202/pY204.22A). This work is in progress.
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The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation. The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
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