Anti-p-ERK 1/2 Antibody (pT202/pY204.22A) recommended for detection of Thr 202 and Tyr 204 dually phosphorylated ERK 1 of human origin, correspondingly dually phosphorylated ERK 1 of mouse and rat origin; and...by WB, IF and IHC(P)

Anti-p-ERK 1/2 Antibody (pT202/pY204.22A): sc-136521

Anti-p-ERK 1/2 Antibody (pT202/pY204.22A) is rated 4.3 out of 5 by 31.
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Datasheets

    • p-ERK 1/2 Antibody (pT202/pY204.22A) is a mouse monoclonal IgG1 κ, cited in 96 publications, provided at 200 µg/ml
    • raised against a short amino acid sequence containing dually phosphorylated Thr 202 and Tyr 204 of ERK 1 of rat origin
    • recommended for detection of Thr 202 and Tyr 204 dually phosphorylated ERK 1 of human origin, correspondingly Thr 203 and Tyr 205 dually phosphorylated ERK 1 of mouse and rat origin; and Thr 185 and Tyr 187 dually phosphorylated ERK 2 of human origin, correspondingly Thr 183 and Tyr 185 dually phosphorylated ERK 2 of mouse and rat origin by WB, IF and IHC(P)
    • available conjugated to agarose for IP; and to HRP for WB, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-ERK 1/2 (pT202/pY204.22A): sc-136521.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-ERK 1/2 Antibody (pT202/pY204.22A). This work is in progress.
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How many amino acids in length is the antigen phosphopeptide of this antibody?

Asked by: tarotaro27
Thank you for your inquiry. This antibody is raised against a short amino acid sequence containing dually phosphorylated Thr 202 and Tyr 204 of ERK 1 of rat origin. Unfortunately we do not known the exactly amino acids sequence of the antigen. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Sen Li
Date published: 2021-11-23

What is the complete amino acid sequence of the antigen phosphopeptide used as a source of this antibody?

Asked by: tarotaro27
Thank you for your inquiry. This antibody is raised against a short amino acid sequence containing dually phosphorylated Thr 202 and Tyr 204 of ERK 1 of rat origin.
Answered by: Sen Li
Date published: 2021-11-16

Can this antibody be used for IHC or IF on mouse tissue samples?

Asked by: Anna99
Unfortunately this antibody is not available directly conjugated for this type of application. See p-ERK (E-4): sc-7383 for p-ERK 1/2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647.
Answered by: Technical Support 7
Date published: 2017-01-12

What is the best secondary to use with this mouse monoclonal p-ERK 1/2 Antibody?

Asked by: Anna99
Instead of a traditional secondary antibody, we recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-12
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Rated 4 out of 5 by from p-ERK 1/2 Antibody (pT202/pY204.22A) I used p-ERK 1/2 Antibody (pT202/pY204.22A)(sc-136521) for Western Blot analysis of human protein smaple. The reults are very good.
Date published: 2020-08-10
Rated 5 out of 5 by from Very satisfied Ordered 3 weeks ago. In my hands, the antibody gives very good signal in 1:1500 dilution (WB).
Date published: 2019-11-16
Rated 5 out of 5 by from Work great on human samples One specific band on Western blot with human samples.
Date published: 2019-07-23
Rated 4 out of 5 by from western blot We detected the expression of pErk1/2 in hepatocellular cancer cells, and got a good results. (1:300-400)
Date published: 2019-03-05
Rated 5 out of 5 by from Tres bon signale specifique ! J'ai testé un échantillons gratuit en WB et il marche très bien.
Date published: 2017-11-28
Rated 1 out of 5 by from Pas de signal détecté dans nos conditions. J'ai testé une première fois cet anticorps (membrane PVDF, dilution AC primaire 1/1000e) mais nous n'avons détecté aucun signal. Nous allons recommencer, en utilisant une membrane de nitrocellulose cette fois ci et en augmentant la concentration de l'anticorps (1/500e).
Date published: 2017-10-17
Rated 4 out of 5 by from negative I have tried many times , and it is still negative results.
Date published: 2017-10-05
Rated 4 out of 5 by from good antibody with brain tissue I got a free sample of this antibody to test in brain tissue with regular IHC protocol, and it worked very well.
Date published: 2017-10-04
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