Anti-p-ERK 1/2 Antibody (12D4) recommended for detection of Thr 202 and Tyr 204 dually phosphorylated ERK 1 and ERK 2 of mouse, rat, human and canine origin by WB, IP and IF

Anti-p-ERK 1/2 Antibody (12D4): sc-81492

Anti-p-ERK 1/2 Antibody (12D4) is rated 4.9 out of 5 by 11.
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Datasheets

    • p-ERK 1/2 Antibody (12D4) is a mouse monoclonal IgG1, cited in 200 publications, provided at 50 µg/0.5 ml
    • raised against synthetic phosphopeptide corresponding to amino acid residues surrounding the T-E-Y motif of ERK1 of human origin
    • recommended for detection of Thr 202 and Tyr 204 dually phosphorylated ERK 1 and ERK 2 of mouse, rat, human and canine origin by WB, IP and IF; non cross-reactive with non-phosphorylated ERK 1/2; requires phosphorylation at both the Thr and Tyr site
    • See p-ERK (E-4): sc-7383 for p-ERK 1/2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-ERK 1/2 (12D4): sc-81492.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-ERK 1/2 Antibody (12D4). This work is in progress.
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How many amino acids in length is the antigen phosphopeptide of this antibody?

Asked by: tarotaro27
Thank you for your inquiry. This antibody is raised against synthetic phosphopeptide corresponding to amino acid residues surrounding the T-E-Y motif of ERK1 of human origin. Unfortunately we do not known the exactly amino acids sequence of the antigen. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Sen Li
Date published: 2021-11-23

What is the complete amino acid sequence of the antigen phosphopeptide used as a source of this antibody?

Asked by: tarotaro27
Thank you for your inquiry. this antobody is raised against a short amino acid sequence containing dually phosphorylated Thr 202 and Tyr 204 of ERK 1 of rat origin.
Answered by: Sen Li
Date published: 2021-11-16

Does this antibody (sc-81492) recognize both dually phosphorylated ERK 1 (thr202 and tyr204) and ERK 2 (thr185 and tyr187)? I have seen only one band in the datasheet.

Asked by: Biologist
Thank you for your question. Yes, this antibody, p-ERK 1/2 (12D4): sc-81492 is recommended for detection of Thr 202 and Tyr 204 dually phosphorylated ERK 1 and ERK 2 of mouse, rat, human and canine origin by WB, IP and IF. It is non cross-reactive with non-phosphorylated ERK 1/2, and requires phosphorylation at both the Thr and Tyr sites for reactivity. ERK1 and ERK 2 are very similar in size, and we do not typically observe a doublet or more than one band when using this antibody in Western blotting.
Answered by: Technical Support
Date published: 2018-04-22

Is p-ERK 1/2 Antibody (12D4): sc-81492 available directly conjugated to HRP or fluorescent dyes?

Asked by: jenni
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using p-ERK (E-4), which is available conjugated to AC, HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. (https://www.scbt.com/scbt/product/p-erk-antibody-e-4)
Answered by: Technical Support 2
Date published: 2018-04-22
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Rated 4 out of 5 by from Great antibody Great bands and specific signal. But I had to load 80μg protein to detect it. Maybe it was due to my cell line (HaCat cells)
Date published: 2021-03-22
Rated 5 out of 5 by from Great antibody It specifically detects the protein of interest. Working dilution:1:200.
Date published: 2019-11-11
Rated 5 out of 5 by from great antibody I like to work with santa cruz antibodies. They work great!
Date published: 2019-11-05
Rated 5 out of 5 by from Excellent antibody Excellent antibody for detection of phosphorylated Erk. No background even with higher dilution
Date published: 2019-03-19
Rated 5 out of 5 by from Outstanding Very clear phospho- specific results, low background
Date published: 2017-01-07
Rated 5 out of 5 by from Dynamite in your WB This antibody offers tried and true results. It will present clear and specific bands of p-ERK. The data is convincing, but over 30 citations are even more so.
Date published: 2016-12-29
Rated 5 out of 5 by from Good Western blot data in analyzing ERK 1/2 Good Western blot data in analyzing ERK 1/2 phosphorylation in untreated, PMA treated, and PMA and lambda protein phosphatase treated Jurkat whole cell lysates. -SCBT QC
Date published: 2015-03-30
Rated 5 out of 5 by from Published with this antibody for Western Published with this antibody for Western blot in C4-2B cells. -SCBT Publication Review
Date published: 2015-03-29
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