p-ERK 1/2 Antibody (12D4) is a mouse monoclonal IgG1, cited in 211 publications, provided at 50 µg/0.5 ml
raised against synthetic phosphopeptide corresponding to amino acid residues surrounding the T-E-Y motif of ERK1 of human origin
recommended for detection of Thr 202 and Tyr 204 dually phosphorylated ERK 1 and ERK 2 of mouse, rat, human and canine origin by WB, IP and IF; non cross-reactive with non-phosphorylated ERK 1/2; requires phosphorylation at both the Thr and Tyr site
See p-ERK (E-4): sc-7383 for p-ERK 1/2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-ERK 1/2 (12D4): sc-81492.
At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-ERK 1/2 Antibody (12D4). This work is in progress.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation. The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.