Anti-p-Cdc2 p34 Antibody (pY15.44) recommended for detection of Tyr 15 phosphorylated Cdc2 p34 of mouse, rat and human origin by WB, IP and IF

Anti-p-Cdc2 p34 Antibody (pY15.44): sc-136014

Anti-p-Cdc2 p34 Antibody (pY15.44) is rated 3.6 out of 5 by 5.
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Datasheets

    • Anti-p-Cdc2 p34 Antibody (pY15.44) is a mouse monoclonal IgG1 κ p-Cdc2 p34 antibody, cited in 8 publications, provided at 200 µg/ml
    • raised against a short amino acid sequence containing phosphorylated Tyr 15 of Cdc2 p34 of human origin
    • recommended for detection of Tyr 15 phosphorylated Cdc2 p34 of mouse, rat and human origin by WB, IP and IF
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Cdc2 p34 (pY15.44): sc-136014.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-Cdc2 p34 Antibody (pY15.44) for WB applications. These reagents are now offered in bundles with p-Cdc2 p34 Antibody (pY15.44) (see ordering information below).
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I am using p-Cdc2 p34 (pY15.44): sc-136014 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Randy McDonald
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 1 out of 5 by from Had bad luck with IF Had received sample of this antibody. This sample did not work for IF. I fixed my cells according to the well described method that involves 4% PFA (we fix at 4 degrees C). I used it in a cocktail of 1:100 dilution with other antibody (also in 1:100 dilution). The other antibody in the cocktail and DAPI worked as fine. Our secondary antibodies are great, so the only thing that I am not sure of is this antibody.
Date published: 2019-03-28
Rated 5 out of 5 by from WB experiments Our laboratory is mainly done WB experiments, this antibody works really well in WB 1:1000 in human colon carcinoma cells.
Date published: 2017-05-22
Rated 4 out of 5 by from Strong blown out band in Saos Strong blown out band in Saos-2 whole cell lysates. -SCBT QC
Date published: 2015-02-19
Rated 4 out of 5 by from Great antibody for IP and Western Great antibody for IP and Western! Works in many species. -SCBT Publication Review
Date published: 2015-01-12
Rated 4 out of 5 by from Detected endogenous Cdc42 in HeLa whole cell Detected endogenous Cdc42 in HeLa whole cell lysates. -SCBT QC
Date published: 2013-06-10
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