Anti-p-CaMKII Antibody (22B1) recommended for detection of Thr 286 phosphorylated CaMKII of mouse, rat and human origin by WB, IP and IF

Anti-p-CaMKII Antibody (22B1): sc-32289

Anti-p-CaMKII Antibody (22B1) is rated 4.7 out of 5 by 15.
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Datasheets

    • p-CaMKII Antibody (22B1) is a mouse monoclonal IgG1 κ, cited in 55 publications, provided at 200 µg/ml
    • raised against a synthetic peptide corresponding to residues 281-294 of CaMKII of rat origin
    • Anti-p-CaMKII Antibody (22B1) is recommended for detection of Thr 286 phosphorylated CaMKII of mouse, rat and human origin by WB, IP and IF
    • Anti-p-CaMKII Antibody (22B1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-CaMKII (22B1): sc-32289.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for p-CaMKII Antibody (22B1) for WB applications. This reagent is now offered in a bundle with p-CaMKII Antibody (22B1) (see ordering information below).
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What is the recommended secondary reagent to use with p-CaMKII (22B1): sc-32289 monoclonal antibody?

Asked by: jenniferc
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2021-11-06
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Rated 5 out of 5 by from 功能不错,挺好用的,希望做出不错的结果 我前段时间买了这个抗体做COIP,没想到很久做不出来的居然有了,挺不错的,这次打算买正装,上次申请的小样
Date published: 2021-06-08
Rated 4 out of 5 by from IHC (Frozen)でワークします。 4%PFA (4℃ overnight)で固定したマウス脳の凍結切片を100倍希釈で染色したところ 抗CAMK2抗体と同様のパターンで染色されました。上記固定条件では抗原賦活化処理を行う方が染色強度が高いです。
Date published: 2020-08-31
Rated 5 out of 5 by from Western blot in Drosophila Thank you for your company free trial sample. This antibody (1:200, 5% skim milk in1x TBST ) was perfect working in Drosophila eye imaginal discs lysate (20 of larvae).
Date published: 2019-09-12
Rated 4 out of 5 by from 试用装还可以 申请的试用装,在小鼠神经干细胞(NE4C细胞系)WB 1:1000能出条带,条带还挺清楚,用的脱脂奶粉封闭
Date published: 2017-10-14
Rated 3 out of 5 by from Not so good 1:200 diluted for wb of rat protein ,the band is not so strong!
Date published: 2017-07-29
Rated 5 out of 5 by from great antibody Western blot in rat brain tissue and it works beautifully.
Date published: 2017-05-13
Rated 5 out of 5 by from strong bands Great band in WB on rat brain tissue, I like it for WB detection
Date published: 2017-04-24
Rated 5 out of 5 by from Ottimo anticorpo Thank you for the opportunity to test your product anti-pCaMKII (22B1) sc-32289, Lot # D0716 mouse monoclonal antibody. We tested p-CaMKII by western blotting analysis. 60 micrograms of mouse brain cytosolic lysates were resolved on 10% SDS-PAGE, and incubated over night with monoclonal pCaMKII sc-32289 antibody (1:1000). Secondary antibody utilized was anti-mouse sc-2005 antibody (1:5000). pCaMK II signal was detected by chemidoc (Biorad) and a good quality of signal was reveled since short exposition. Based on our experience we recommended pCaMKII sc-32289 product for further studies (5 stars).
Date published: 2017-04-05
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