Anti-p-c-Src/Src Antibody (H-3) recommended for detection of Tyr 530 phosphorylated c-Src of human and rat origin, and Tyr 535 phosphorylated c-Src of mouse origin by WB, IP, IF, IHC(P) and ELISA

Anti-p-c-Src/Src Antibody (H-3): sc-166860

Anti-p-c-Src/Src Antibody (H-3) is rated 4.7 out of 5 by 18.
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Datasheets

    • p-c-Src/Src Antibody (H-3) is a mouse monoclonal IgG2b κ, cited in 18 publications, provided at 200 µg/ml
    • p-c-Src/Src Antibody (H-3) is recommended for detection of Tyr 530 phosphorylated c-Src of human and rat origin, and Tyr 535 phosphorylated c-Src of mouse origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-p-c-Src/Src Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-c-Src/Src (H-3): sc-166860.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-c-Src/Src Antibody (H-3) for WB and IHC(P) applications. These reagents are now offered in bundles with p-c-Src/Src Antibody (H-3) (see ordering information below).
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What application is the blocking peptide sc-166860 P appropriate for?

Asked by: jenniferc
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Worked for human tissue samples WB Worked well for human colon cancer cell lysates using WB at 1:200. Had to expose for longer period of time though.
Date published: 2018-03-22
Rated 4 out of 5 by from p-c-Src Antibody(H-3) H-3 is the ideal ab to detect p-c-Src of my sample, but it needs high concentration to start with.
Date published: 2018-03-07
Rated 5 out of 5 by from best phospho src use in a experiment of WB. it works very well for me
Date published: 2018-02-21
Rated 3 out of 5 by from seems not good for liver sample test I try to test healthy liver samples, but seems only a very specific band with about 38Kd, but no the target band.
Date published: 2017-12-04
Rated 5 out of 5 by from Good antibody Gives me strong and specific bands. I would recommend it.
Date published: 2017-09-23
Rated 5 out of 5 by from works great on western blot and IHC We have used this antibody on human skin tissue. It works great on both western blot and IHC.
Date published: 2017-09-20
Rated 5 out of 5 by from sharp bands This antibody was tested on human aortic endothelial cells post various treatments. It gives specific band with no background. A non-specific band around 40 kDa was observed.
Date published: 2017-09-14
Rated 5 out of 5 by from there is some non-specific binding the background is clear,but there is some non-specific bindind
Date published: 2017-08-25
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