p-Annexin II Antibody (85.Tyr 24)recommended for detection of Tyr 24 phosphorylated Annexin II of mouse, rat and human origin by WB, IP and ELISA

p-Annexin II Antibody (85.Tyr 24): sc-135753

p-Annexin II Antibody (85.Tyr 24) is rated 4.5 out of 5 by 4.
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Datasheets
    • p-Annexin II Antibody (85.Tyr 24) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against a short amino acid sequence containing phosphorylated Tyr 24 of Annexin II of human origin
    • recommended for detection of Tyr 24 phosphorylated Annexin II of mouse, rat and human origin by WB, IP and ELISA
    • available conjugated to agarose for IP; and to HRP for WB, IHC(P) and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Annexin II (85.Tyr 24): sc-135753.
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做ICC的稀释比例是多少,可以用PBS洗吗?还是得用TBST

Asked by: xiaoman123
感谢您的提问。p-Annexin II 抗体(85.Tyr 24)不推荐ICC的实验方法。对于ICC实验流程,详见链接:https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining. Thank you for your inquiry. p-Annexin II Antibody (85.Tyr 24) is not recommended for ICC application. For ICC protocol, see the link: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining.
Answered by: Technical Support 9
Date published: 2019-05-22

What is the total volume provided of p-Annexin II (85.Tyr 24): sc-135753 monoclonal antibody?

Asked by: jenni
Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 15
Date published: 2017-01-25
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Rated 4 out of 5 by from Works Well Seems to work well for both Westerns and Immunofluorescence.
Date published: 2018-07-27
Rated 5 out of 5 by from Popular choice! This clone is very sensitive and a very popular choice for detection of Annexin II phosphorylated at Tyrosine 24 by WB, IF and IHC as indicated by the recent product citations!
Date published: 2017-01-13
Rated 5 out of 5 by from Positive detection of phosphorylated Annexin Positive detection of phosphorylated Annexin II by WB using K562 whole cell lysate. -SCBT QC
Date published: 2015-01-12
Rated 4 out of 5 by from Able to get great signal with 1:2000 dilution Able to get great signal with 1:2000 dilution with WB.
Date published: 2015-01-05
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