Anti-p-Akt1 Antibody (5.Ser 473) recommended for detection of Ser 473 phosphorylated Akt1 of mouse, rat and human origin by WB, IP and ELISA

Anti-p-Akt1 Antibody (5.Ser 473): sc-293125

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Anti-p-Akt1 Antibody (5.Ser 473) is rated 4.4 out of 5 by 15.
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Datasheets

    • p-Akt1 Antibody (5.Ser 473) is a mouse monoclonal IgG1 κ, cited in 103 publications, provided at 200 µg/ml
    • raised against a short amino acid sequence containing phosphorylated Ser 473 of Akt1 of human origin
    • recommended for detection of Ser 473 phosphorylated Akt1 of mouse, rat and human origin by WB, IP and ELISA; may cross-react with Ser 473 phosphorylated Akt2 and Ser 473 phosphorylated Akt3
    • available conjugated to agarose for IP; and to HRP for WB, IHC(P) and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Akt1 (5.Ser 473): sc-293125.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-Akt1 Antibody (5.Ser 473) for WB applications. These reagents are now offered in bundles with p-Akt1 Antibody (5.Ser 473) (see ordering information below).
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What is the exact MW of phospho Akt and phospho ERK? Sc 7985R and Sc 101 760 catalog number, respectively. Rabbit polyclonal

Asked by: Marialice
Thank you for your question. The expected molecular weight for these phosphorylated proteins is published on our antibody data sheets. You can still download the data sheets for these now discontinued antibodies by searching on our website with the Data Sheet search, or use the link below: https://www.scbt.com/resources/datasheet-search
Answered by: Technical Support
Date published: 2020-03-23

What secondary is best to use with p-Akt1 (5.Ser 473): sc-293125?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with p-Akt1 (5.Ser 473): sc-293125 monoclonal antibody, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-17
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