Anti-p-Akt1 Antibody (104A282) recommended for detection of Ser 473 phosphorylated Akt1 of mouse, rat and human origin by WB and IP

Anti-p-Akt1 Antibody (104A282): sc-52940

Anti-p-Akt1 Antibody (104A282) is rated 4.7 out of 5 by 7.
  • y_2021, m_11, d_29, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_en_US, sid_52940, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 129ms
  • REVIEWS, PRODUCT
loading
5
5
4
2
3
0
2
0
1
0

Datasheets

    • p-Akt1 Antibody (104A282) is a mouse monoclonal IgG1 κ, cited in 31 publications, provided at 100 µg/ml
    • raised against synthetic p-Ak1 of human origin
    • recommended for detection of Ser 473 phosphorylated Akt1 of mouse, rat and human origin by WB and IP
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Akt1 Antibody (104A282). This work is in progress.
loading

What secondary antibody would you recommend using with this products?

Asked by: cmo97
Thank you for your question. p-Akt1 Antibody (104A282) is a mouse monoclonal IgG1 (with kappa light chain), and we would recommend using our mouse IgG kappa binding protein (m-IgGκ BP) as a secondary antibody. A complete list of available conjugates can be found below. https://www.scbt.com/scbt/browse/Secondary-IgGs-and-IgG-Binding-Proteins/_/N-1hoaxk4
Answered by: Technical Support
Date published: 2019-06-18

I am using p-Akt1 (104A282): sc-52940 antibody for immunoprecipitation, how much antibody should I use?

Asked by: jerojero
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
  • y_2021, m_11, d_29, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_52940, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 111ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from great antibody I bought it and it worked excellent. I like to work with santa cruz antibody.
Date published: 2019-11-05
Rated 4 out of 5 by from Good antibodies The antibodies works good. I used this antibody 1:1000 on Western blot.
Date published: 2018-06-29
Rated 4 out of 5 by from Worked with human transfected lysate by WB Worked with human transfected lysate by WB. -SCBT QC
Date published: 2015-04-05
Rated 5 out of 5 by from WB publication data in A431 and HeLa samples WB publication data in A431 and HeLa samples. -SCBT Publication Review
Date published: 2015-03-09
Rated 5 out of 5 by from Good WB data using Jurkat whole cell lysates Good WB data using Jurkat whole cell lysates. -SCBT QC
Date published: 2015-03-07
Rated 5 out of 5 by from Great WB publication with A549 Great WB publication with A549, H157 and H520 samples. -SCBT Publication Review
Date published: 2015-02-11
Rated 5 out of 5 by from Antibody works great for WB with 3T3 Antibody works great for WB with 3T3-L1 whole cell lysates. -SCBT QC
Date published: 2015-02-08
  • y_2021, m_11, d_29, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_7
  • loc_en_US, sid_52940, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT