Anti-p-Akt1/2/3 Antibody (A-12) recommended for detection of Thr 308 phosphorylated Akt1 and correspondingly Thr 309 phosphorylated Akt2 and correspondingly Thr 305 phosphorylated Akt3 of mouse, rat, human and origin by WB, IP, IF and ELISA

Anti-p-Akt1/2/3 Antibody (A-12): sc-271964

Anti-p-Akt1/2/3 Antibody (A-12) is rated 4.5 out of 5 by 4.
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Datasheets

    • p-Akt1/2/3 Antibody (A-12) is a mouse monoclonal IgM κ, cited in 17 publications, provided at 200 µg/ml
    • epitope corresponding to a short amino acid sequence containing phosphorylated Thr 308/309/305 of Akt2 of human origin
    • recommended for detection of Thr 308 phosphorylated Akt1 and correspondingly Thr 309 phosphorylated Akt2 and correspondingly Thr 305 phosphorylated Akt3 of mouse, rat, human and origin by WB, IP, IF and ELISA; also reactive with additional species, including equine and avian
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Akt1/2/3 Antibody (A-12). This work is in progress.
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What application is the blocking peptide sc-271964 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good antibodies The antibodies works good. I used this antibody 1:1000 on Western blot.
Date published: 2018-06-29
Rated 5 out of 5 by from Great antibody! I used this antibody for the WB with the protein extraced from H9C2 cell,and got a clear and special band !
Date published: 2017-02-15
Rated 5 out of 5 by from Used this antibody in WB in human cancer Used this antibody in WB in human cancer cell lines and got clean blot with minimal background.
Date published: 2015-10-20
Rated 4 out of 5 by from Publishable results in WB with human SH Publishable results in WB with human SH- SY5Y cells. -SCBT Publication Review
Date published: 2015-02-21
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