Anti-p-Akt1/2/3 Antibody (11E6) recommended for detection of Ser 473 phosphorylated Akt1 and correspondingly Ser 474 phosphorylated Akt2 and Ser 472 phosporylated Akt3 of mouse, rat, human and canine origin by WB and IP

Anti-p-Akt1/2/3 Antibody (11E6): sc-81433

Anti-p-Akt1/2/3 Antibody (11E6) is rated 4.8 out of 5 by 13.
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Datasheets

    • p-Akt1/2/3 Antibody (11E6) is a mouse monoclonal IgG1, cited in 73 publications, provided at 50 µg/0.5 ml
    • raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding serine 473 of Akt1 of human origin
    • recommended for detection of Ser 473 phosphorylated Akt1 and correspondingly Ser 474 phosphorylated Akt2 and correspondingly Ser 472 phosporylated Akt3 of mouse, rat, human and canine origin by WB and IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Akt1/2/3 (11E6): sc-81433.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-Akt1/2/3 Antibody (11E6) for WB applications. These reagents are now offered in bundles with p-Akt1/2/3 Antibody (11E6) (see ordering information below).
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FREE 10 µg sample of p-Akt1/2/3 (11E6): sc-81433.

Asked by: akki
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: Tech Service
Date published: 2019-01-07

I am using p-Akt1/2/3 Anticuerpo (11E6): sc-81433 for Western Blot. We have done the test with BSA and milk and it has not worked. Do you know what is better to perform the blockage and incubation?

Asked by: Azahara
Muchas gracias por su pregunta. Para Western Blot le sugerimos que use éste anticuerpo a una dilución inicial de 1: 200, incubándolo durante la noche a 4°C. El bloqueo de la membrana debe realizarse con una solución que contenga un 5% de leche y un 0.05% Tween20 en TBS durante 60 minutos a temperatura ambiente. El gel debe cargarse con 50 µg de lisado total en cada pocillo. Por favor encuentre toda la información disponible sobres este protocolo: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support Europe
Date published: 2017-12-19

Is this antibody specific for the phosphorylated protein, and will not cross-react with the non-phosphorylated form of the protein?

Asked by: TinTin
Yes. p-Akt1/2/3 (11E6): sc-81433 is specific for phosphorylated Akt1/2/3. The antibody has been validated through a series Western blot experiments using lambda phosphatase treated lysates to demonstrate phospho-specificity.
Answered by: Tech Service
Date published: 2017-01-30
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Rated 5 out of 5 by from Effects of genistein and estradiol on hepatocytes Genistein/17β-estradiol protected hepatocytes against NAFLD-like medium, by preventing the loss of cell viability and mitochondrial function, triglycerides accumulation and peroxidation. The blocking of kinases, ERs and GPER was able to reduce the above effects, which were potentiated by NLRP3 inflammasome. The results shown activation of Akt in hepatocytes in response to genistein and estradiol.
Date published: 2021-02-04
Rated 5 out of 5 by from Very good for WB We used it at 1:500 dilution and it specifically detected endogenous human pAKT1/2/3.
Date published: 2020-06-25
Rated 5 out of 5 by from Excellent service! I bought this primary antibody and received other trial sample antibodies. The customer service were excellent
Date published: 2019-02-01
Rated 4 out of 5 by from Good antibodies I got a free sample from company. It works in dilution 1:1000 (WB)
Date published: 2018-06-10
Rated 4 out of 5 by from Good antibodies I got a free sample for company. I used this antibodies for WB, 1:1000 dilution. Antibody worked good.
Date published: 2018-04-19
Rated 4 out of 5 by from No protein bands I didn't get any bands, but I'm not sure the reason.
Date published: 2018-04-02
Rated 5 out of 5 by from Good WB data with Mouse liver this antibody worked very well with Mouse liver,I got the good bands and it is my desired
Date published: 2017-01-10
Rated 5 out of 5 by from Produced publishable WB data on mouse and human Great established p-akt1/2/3 ab with a dozen citations in the primary literature. Well characterized and strong WB banding
Date published: 2017-01-09
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