p-4E-BP1/2/3 Antibody (A-10)recommended for detection of Thr 45 phosphorylated 4E-BP1 and correspondingly phosphorylated 4E-BP2 and 4E-BP3 of mouse, rat and human origin by WB, IP, IF and ELISA

p-4E-BP1/2/3 Antibody (A-10): sc-271947

p-4E-BP1/2/3 Antibody (A-10) is rated 4.8 out of 5 by 6.
  • y_2019, m_9, d_16, h_7
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.13
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_271947, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 238ms
  • REVIEWS, PRODUCT
Datasheets
    • p-4E-BP1/2/3 Antibody (A-10) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • p-4E-BP1/2/3 Antibody (A-10) is recommended for detection of Thr 45 phosphorylated 4E-BP1 and correspondingly phosphorylated 4E-BP2 and 4E-BP3 of mouse, rat and human origin by WB, IP, IF and ELISA
    • p-4E-BP1/2/3 Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-271947 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-4E-BP1/2/3 (A-10): sc-271947.
Submit a review for this product and receive 15 CruzCredits

What application is the blocking peptide sc-271947 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-25
  • y_2019, m_9, d_16, h_7CST
  • bvseo_bulk, prod_bvqa, vn_bulk_2.0.13
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_271947, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 235ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from hard it's hard for me to detect this protein by using this antibody.
Date published: 2017-10-05
Rated 5 out of 5 by from western blot i asked the sample for 3T3-l1 cell western blot , work very well, clear band.
Date published: 2017-09-27
Rated 5 out of 5 by from Good results This antibody gave satisfying results in wetern blot.
Date published: 2017-09-05
Rated 5 out of 5 by from Excellent This antibody works well when we use 1:500 dilution in WB, and our results shows a beautiful brand.
Date published: 2017-03-21
Rated 5 out of 5 by from Very potent and specific antibody This monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and is highly recommended for Western blotting. There was very strong detection of 4E-BP1 in overnight serum-starved human A431 cells treated for 5 minutes with EGF on Western blots, and no significant cross-reactivities on these blots with any off-target proteins.
Date published: 2017-03-14
Rated 5 out of 5 by from Produced positive Western Blot data of 4E Produced positive Western Blot data of 4E-BP1/2/3 phosphorylation in untreated, calyculin A treated and calyculin A and lambda protein phosphatase treated Jurkat whole cell lysates. -SCBT QC
Date published: 2013-09-08
  • y_2019, m_9, d_16, h_7
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.13
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_6
  • loc_en_US, sid_271947, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 108ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.