Anti-OPG/Osteoprotegerin Antibody (4H219) recommended for detection of OPG of human origin by WB and IP

Anti-OPG/Osteoprotegerin Antibody (4H219): sc-71747

Anti-OPG/Osteoprotegerin Antibody (4H219) is rated 4.0 out of 5 by 2.
  • y_2021, m_7, d_25, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_71747, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 150ms
  • REVIEWS, PRODUCT
loading
5
0
4
2
3
0
2
0
1
0

Datasheets

    • OPG/Osteoprotegerin Antibody (4H219) is a mouse monoclonal IgG1, cited in 4 publications, provided at 50 µg/0.5 ml
    • raised against amino acids 20-37 of OPG of human origin
    • recommended for detection of OPG of human origin by WB and IP
    • See OPG/Osteoprotegerin (E-10): sc-390518 for OPG/Osteoprotegerin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for OPG/Osteoprotegerin Antibody (4H219). This work is in progress.
loading

I am using OPG (4H219): sc-71747 antibody for immunoprecipitation, how much antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
  • y_2021, m_7, d_25, h_19CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_71747, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 122ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Good specificity in WB in human transfected Good specificity in WB in human transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-01-28
Rated 4 out of 5 by from WB publishable data with human GC cell lines WB publishable data with human GC cell lines - SCBT Publication Review
Date published: 2014-07-15
  • y_2021, m_7, d_25, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.18
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_en_US, sid_71747, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 15ms
  • REVIEWS, PRODUCT