Anti-Omni-probe Antibody (D-8) recommended for detection of fusion proteins encoded by the prokaryotic pRSET A, B, C & pTrc His A, B, C; baculovirus pBlue Bac His A, B, C & mammalian pEBV His A, B, C expression vectors by WB & IP

Anti-Omni-probe Antibody (D-8): sc-7270

Anti-Omni-probe Antibody (D-8) is rated 4.6 out of 5 by 10.
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Datasheets

    • Anti-Omni-probe Antibody (D-8) is a mouse monoclonal IgG2a κ Omni-probe antibody, cited in 57 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 8-30 within the (His)6 and polylinker sequence
    • Omni-probe Antibody (D-8) is recommended for detection of fusion proteins encoded by the prokaryotic pRSET A, B, C and pTrc His A, B, C; baculovirus pBlue Bac His A, B, C and mammalian pEBV His A, B, C expression vectors by WB and IP
    • Anti-Omni-probe Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Omni-probe (D-8): sc-7270.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Omni-probe Antibody (D-8) for WB applications. These reagents are now offered in bundles with Omni-probe Antibody (D-8) (see ordering information below).
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What is your recommended western blotting secondary antibody for use with Omni-probe (D-8): sc-7270?

Asked by: jenniferc
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-02-04
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Rated 5 out of 5 by from Excellent choice for Omni-probe detection This mouse monoclonal (D-8) clone is an excellent choice for detection of Omni-probe labeled proteins. It has been featured in over 30 journal articles!
Date published: 2017-01-19
Rated 5 out of 5 by from Good Western bands detected with Omni Good Western bands detected with Omni-tag fusion proteins -SCBT QC
Date published: 2015-07-05
Rated 4 out of 5 by from Nice publishable data on Western blot on Nice publishable data on Western blot on canine MDCK, D-17 and Cf2Th cells . -SCBT Publication Review
Date published: 2015-06-30
Rated 5 out of 5 by from Published data for IF in human and mouse Published data for IF in human and mouse HEK293 and MCF-7 cells. -SCBT Publication Review
Date published: 2015-06-30
Rated 5 out of 5 by from Published IHC(P) data for use in mouse C4 Published IHC(P) data for use in mouse C4-HD and C4-HI tumors. -SCBT Publication Review
Date published: 2015-06-19
Rated 5 out of 5 by from Published data for IP and WB in canine MKN28 Published data for IP and WB in canine MKN28, AGS, COS-7, and MDCK (MDCK II) cells. -SCBT Publication Review
Date published: 2015-04-17
Rated 4 out of 5 by from Performs well in Western Performs well in Western, good signal with human and mouse BP-A31 and COS-7 cells. -SCBT Publication Review
Date published: 2015-04-16
Rated 4 out of 5 by from WB: Worked well in mouse NIH 3T3 cells WB: Worked well in mouse NIH 3T3 cells. -SCBT Publication Review
Date published: 2015-03-26
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