Anti-Oct-3/4 Antibody (C-10) is a mouse monoclonal IgG2b κ Oct-3/4 antibody, cited in 2,233 publications, provided at 200 µg/ml
raised against amino acids 1-134 of Oct-3/4 of human origin
Anti-Oct-3/4 Antibody (C-10) is recommended for detection of Oct-3/4 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; non cross-reactive with Oct-3/4 isoform B
Anti-Oct-3/4 Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to biotin for WB, IHC(P) and ELISA
TransCruz reagent for Gel Supershift and ChIP applications (sc-5279 X, 200 µg/0.1 ml)
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Oct-3/4 (C-10): sc-5279.
m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Oct-3/4 Antibody (C-10) for WB and IHC(P) applications. These reagents are now offered in bundles with Oct-3/4 Antibody (C-10) (see ordering information below).
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possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Oct-3/4 Antibody (C-10) is a high quality monoclonal Oct-3/4 antibody (also designated OCT4 antibody or POU5F1 antibody) suitable for the detection of the Oct-3/4 protein of mouse, rat and human origin. Oct-3/4 Antibody (C-10) is available as both the non-conjugated anti-Oct-3/4 antibody form, as well as multiple conjugated forms of anti-Oct-3/4 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. POU5F1 (POU domain, class 5, transcription factor 1), also known as octamer-binding transcription factor-3 (Oct-3, Otf-3), octamer-binding transcription factor-4 (Oct-4, Otf-4) and Oct-3/4, modulates embryonic stem (ES) cell populations by influencing lineage commitment. Oct-3/4 sustains stem-cell self-renewal and differentiation pathways. Transcription factors containing the POU homeodomain regulate tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Oct-3/4 is capable of inducing rapid proliferation and tumorigenic properties of ES cells through activation of the UTF1 gene. In humans, two Oct-3/4 isoforms contribute to influencing the undifferentiated phenotype of ES cells. Oct-3/4 pseudogenes localizing to human chromosomes 10 and 8 are reported to be transcribed in certain cancer cell lines and tissues.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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