Anti-Oct-3/4 Antibody (C-10) recommended for detection of Oct-3/4 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

Anti-Oct-3/4 Antibody (C-10): sc-5279

Anti-Oct-3/4 Antibody (C-10) is rated 4.5 out of 5 by 57.
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Datasheets

    • Anti-Oct-3/4 Antibody (C-10) is a mouse monoclonal IgG2b κ Oct-3/4 antibody, cited in 2,233 publications, provided at 200 µg/ml
    • raised against amino acids 1-134 of Oct-3/4 of human origin
    • Anti-Oct-3/4 Antibody (C-10) is recommended for detection of Oct-3/4 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; non cross-reactive with Oct-3/4 isoform B
    • Anti-Oct-3/4 Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-5279 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Oct-3/4 (C-10): sc-5279.
    • m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Oct-3/4 Antibody (C-10) for WB and IHC(P) applications. These reagents are now offered in bundles with Oct-3/4 Antibody (C-10) (see ordering information below).
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does this antibody cross react with marmoset sample?

Asked by: diksha12
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext .1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2021-09-27

Can I use this antibody for the detection of variant 1 and 2 of OCT3/4?

Asked by: CEDA
Thank you for your question. This antibody is reactive with Oct-3/4, which is Oct 3A. It is non cross-reactive with Oct-3/4 isoform B.
Answered by: Tech Service
Date published: 2019-11-25

Hello, Any good positive control tissue for IHC ?

Asked by: t1234
Thank you for your question. There are many good positive controls for Oct-3/4, here are a few possibilities: adrenal gland, embryonic cells, amnoitic fluid, gonads, brain cells and colorectal cancer.
Answered by: Tech Service
Date published: 2021-08-17

Can you please brief about the antibody diluting solutions suitable for these antibodies with recommended working dilutions? 1. sc-5279 2. sc365823 3. sc8033 4. sc516102

Asked by: medha2001
Thank you for your question. Here is a link to our recommended protocols. You can find all recommended diluents and conditions in the desired protocol. The recommended starting dilutions can also be found on the datasheet that was provided with your antibodies. https://www.scbt.com/scbt/resources/protocols
Answered by: Tech Service
Date published: 2019-08-29

DOES THIS MONOCLONAL REACT WITH CANINE TISSUE?

Asked by: anju1983
Thank you for your question. We have not tested this antibody for reactivity in canine tissue. However, we are aware of at least 2 product citations using the antibody in canine samples. Please refer to PMID’s: 28693187 and 18039902.
Answered by: Technical Support
Date published: 2020-06-08

does this monoclonal react with canine tissue? SC-5279?

Asked by: anjan204
Thank you for your question. We are currently aware of 2 product citations using this antibody with canine tissue. Please refer to PMID’s: 28693187 and 18039902 for more information.
Answered by: Technical Support
Date published: 2019-03-10

Asking about optimal dilution for sc-5279 in lhc-p &if it need heat premeditated

Asked by: Afaq
Thank you for your question. Oct-3/4 Antibody (C-10): sc-5279 is recommended for IHC(P) at a dilution of 1:50-1:500. Our recommended protocol for IHC can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining Please contact our technical service if you have further questions.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

c-10 sc-5279 data sheets say "also available conjugated to biotin for WB, IHC(P) and ELISA". But not clear which one is the biotynilated product, is this Oct-3/4 Antibody (C-10) B sc-5279 B? Thank you

Asked by: eeee
Thank you for your question. The product code for biotinylated Oct-3/4 Antibody (C-10) is sc-5279 B.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29
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Rated 3 out of 5 by from good antibody Signal/noise seems good, but I feel fluorescent signal intensity is weaker than other alternatives. It could be used at a higher concentration. hiPS cell 1:50 dilution 4℃ O/N incubation
Date published: 2021-08-05
Rated 5 out of 5 by from Works well on mouse embryonic stem cells I used this antibody at 0.8 ug/ml,4°C,ON (blocking buffer : PBS + 3% BSA + 0.1% TritonX-100) on mESC fixed with 4% PFA and it shows a very strong nuclear signal. The antibody was received in 2017 and it still works 4 years later.
Date published: 2021-06-04
Rated 5 out of 5 by from worth the price.. doesn't recognize pseudogenes Old lot of this antibody still working. This antibody doesn't recognize pseudogenes and is specific in nature
Date published: 2021-02-17
Rated 5 out of 5 by from SC Oct4 antibody used in 33378662 Great antibody, it works very well on mouse embryonic samples
Date published: 2021-02-15
Rated 5 out of 5 by from great IF antibody I used this antibody for mouse ESC staining to check its pluripotency. It always works well.
Date published: 2021-02-13
Rated 5 out of 5 by from Great antibody for human OCT4 IF This is our standard go to for IF in whole human embryos and human stem cells. Here we have used it successfully in the human embryo and it is also very good for human embryonic stem cells.
Date published: 2020-08-11
Rated 5 out of 5 by from Great antibody SC-5279 worked very well for pluripotency factor oct-4 detection.
Date published: 2019-08-01
Rated 1 out of 5 by from result I used 【ct-3/4 Antibody (C-10): sc-5279】antibody(1:100) for IF of hMSCs , not only the nucleus but also cytoplasm show the same intensity of fluorescence,I don’t know why....
Date published: 2019-07-27
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