Anti-Oct-3/4 Antibody (A-9) recommended for detection of Oct-3/4 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-Oct-3/4 Antibody (A-9): sc-365509

Anti-Oct-3/4 Antibody (A-9) is rated 5.0 out of 5 by 4.
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Datasheets

    • Oct-3/4 Antibody (A-9) is a mouse monoclonal IgG1 λ, cited in 20 publications, provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 16-45 near the N-terminus of Oct-3/4 of human origin
    • Anti-Oct-3/4 Antibody (A-9) is recommended for detection of Oct-3/4 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine and porcine
    • TransCruz reagent for ChIP application (sc-365509 X, 200 µg/0.1 ml)
    • Anti-Oct-3/4 Antibody (A-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Oct-3/4 (A-9): sc-365509.
    • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for Oct-3/4 Antibody (A-9) for WB applications. This reagent is now offered in a bundle with Oct-3/4 Antibody (A-9) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Does this antibody cross-react with OCT4B?

Asked by: timmy1027
Thank you for your question. Based on N-terminal antigenic sequence used to develop this mouse monoclonal antibody, we would not expect it to recognize OCT4B, also known as OCT4B-265, based on the published RefSeq protein sequence on NCBI for this isoform: NP_001272916.1 POU domain, class 5, transcription factor 1 isoform 3.
Answered by: Technical Support
Date published: 2020-11-24

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 6
Date published: 2017-01-20
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Rated 5 out of 5 by from Great WB data Very clean western blot data; strong signal, low background
Date published: 2017-02-05
Rated 5 out of 5 by from Looking for an Oct-3/4 antibody? Excellent choice for detecting Oct-3/4 in both mouse and human samples. Highly recommend this clone!
Date published: 2016-12-30
Rated 5 out of 5 by from The antibody works well for Western blotting The antibody works well for Western blotting detecting endogenous Oct-3/4 from NTERA-2 cl.D1 whole cell lysate. -SCBT QC
Date published: 2015-04-10
Rated 5 out of 5 by from Good antibody for western Good antibody for western, clean background and specific band in F9 whole cell lysate. -SCBT QC
Date published: 2013-06-03
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