Anti-Oct-1 Antibody (12F11) recommended for detection of Oct-1 of mouse, rat, human and Xenopus laevis origin by WB, IP, IF and IHC(P)

Anti-Oct-1 Antibody (12F11): sc-8024

Anti-Oct-1 Antibody (12F11) is rated 4.4 out of 5 by 9.
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Datasheets

    • Anti-Oct-1 Antibody (12F11) is a mouse monoclonal IgG1 κ Oct-1 antibody, cited in 40 publications, provided at 200 µg/ml
    • raised against amino acids 462-760 of Oct-1 of Xenopus laevis origin
    • Anti-Oct-1 Antibody (12F11) is recommended for detection of Oct-1 of mouse, rat, human and Xenopus laevis origin by WB, IP, IF and IHC(P)
    • Anti-Oct-1 Antibody (12F11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-8024 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Oct-1 (12F11): sc-8024.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Oct-1 Antibody (12F11) for WB and IHC(P) applications. These reagents are now offered in bundles with Oct-1 Antibody (12F11) (see ordering information below).
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When should the TransCruz reagent sc-8024 X be used instead of the standard form sc-8024?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from nice band the antibody was tested on rat tissue samples and gave a nice band in western blot.
Date published: 2017-12-13
Rated 5 out of 5 by from it works well for WB I use it for WB RAW264.7 cells cell lysis used. 1:300
Date published: 2017-11-06
Rated 2 out of 5 by from 抗体不是很好 我大约一个星期多前申请的小样,用于做western的,在SKOV3和HO8910上过表达后检测oct1表达量,结果抗体条带强曝光10min条带非常浅,有多条非特异性杂带。总体而言抗体不是很好
Date published: 2017-09-21
Rated 5 out of 5 by from Excellent! This antibody is great in western blot analysis of Oct-1 expression in Ramos whole cell lysate.
Date published: 2017-02-08
Rated 5 out of 5 by from western blot of mouse Western blot of mouse whole cell lysates detect multiple isoforms, the result is consistent with the literature
Date published: 2017-01-03
Rated 4 out of 5 by from Single Strong band for WB in mouse Oct Single Strong band for WB in mouse Oct-1 transfected 293 whole cell lysates.
Date published: 2015-02-27
Rated 5 out of 5 by from Good nuclear staining with formalin Good nuclear staining with formalin-fixed, paraffin-embedded human lymphoma. -SCBT QC
Date published: 2015-01-07
Rated 5 out of 5 by from Good nuclear and cytoplasmic staining of Good nuclear and cytoplasmic staining of decidual cells in paraffin-embedded human placenta tissue showing. -SCBT QC
Date published: 2015-01-03
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