Anti-Oatp2 Antibody (A-2) recommended for detection of Oatp2 of mouse and rat origin by WB, IP, IF and ELISA

Anti-Oatp2 Antibody (A-2): sc-376424

Anti-Oatp2 Antibody (A-2) is rated 4.8 out of 5 by 4.
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Datasheets

    • Anti-Oatp2 Antibody (A-2) is a mouse monoclonal IgG1 κ Oatp2 antibody, cited in 4 publications, provided at 200 µg/ml
    • raised against amino acids 611-660 mapping near the C-terminus of Oatp2 of mouse origin
    • Anti-Oatp2 Antibody (A-2) is recommended for detection of Oatp2 of mouse and rat origin by WB, IP, IF and ELISA
    • Anti-Oatp2 Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Oatp2 (A-2): sc-376424.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for Oatp2 Antibody (A-2) for WB applications. This reagent is now offered in a bundle with Oatp2 Antibody (A-2) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Are there any issues with using sc-376424: Oatp2 (A-2) mouse monoclonal primary antibody on mouse samples?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-376424 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 4 out of 5 by from Oatp2 in mouse liver WCL Conditions: 60 ug protein/lane, 10% gel, no BME dye. Primary concentration, 1:250, incubated at 4C overnight. Secondary concentration, 1:10k, incubated 1 hr at RT. Picture taken with ChemiDoc imager using femto substrate. Strong band appears at about 100 kDa. However, there also appears to be some strong nonspecific bands below 70 kDa.
Date published: 2017-10-26
Rated 5 out of 5 by from Good Antibody Oatp2 Antibody is very fruitful,we usually use the dilution 1:500 and a clean band can be seen.
Date published: 2017-10-24
Rated 5 out of 5 by from Nice antibody Oatp2 Antibody can be used for analyzing samples from mouse, high specificity is the advantage.The analysis results of WB are very intense and vivid.
Date published: 2017-10-21
Rated 5 out of 5 by from Produced positive Western blot data of Oatp2 Produced positive Western blot data of Oatp2 expression in Ramos whole cell lysate. -SCBT QC
Date published: 2014-11-01
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