O-GlcNAc transferase Antibody (C-10)recommended for detection of O-GlcNAc transferase of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA

O-GlcNAc transferase Antibody (C-10): sc-376253

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Datasheets

    • O-GlcNAc transferase Antibody (C-10) is a mouse monoclonal IgG2a (lambda light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 927-961 within an internal region of O-GlcNAc transferase of human origin
    • recommended for detection of O-GlcNAc transferase of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
    • blocking peptide, sc-376253 P
    • See m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See O-GlcNAc transferase (F-12): sc-74546 for O-GlcNAc transferase antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What application is the blocking peptide sc-376253 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Nice monoclonal antibody with western blot We used this antibody for expression detection with western blot. We got a nice band at the correct molecular weight
Date published: 2017-01-31
Rated 5 out of 5 by from Great immunofluorescence nuclear and cytoplasmic Great immunofluorescence nuclear and cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-03-18
Rated 5 out of 5 by from Strong band of interest observed in WB in SK Strong band of interest observed in WB in SK-N-MC nuclear extract. -SCBT QC
Date published: 2015-01-25
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