NuMA Antibody (F-11)recommended for detection of NuMA of human origin by WB, IP, IF, IHC(P) and ELISA

NuMA Antibody (F-11): sc-365532

NuMA Antibody (F-11) is rated 4.5 out of 5 by 6.
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Datasheets

    • NuMA Antibody (F-11) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1816-2115 mapping at the C-terminus of NuMA of human origin
    • NuMA Antibody (F-11) is recommended for detection of NuMA of human origin by WB, IP, IF, IHC(P) and ELISA
    • NuMA Antibody (F-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NuMA (F-11): sc-365532.
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what is the isoelectric point of NUMA(F-11)

Asked by: htting
Thank you for your question. We do not characterize the isoelectric point for any of our antibodies. Although we do not characterize the isoelectric point of our antibodies, we can find some theoretical information regarding isoelectric point. The isoelectric point (pI) of IgG is found in the range of 6.4 to 9.0 [1]. References: [1] Li G, Stewart R, Conlan B, Gilbert A, Roerth P, Nair H. Purification of human immunoglobulin G: A new approach to plasma fractionation. Vox Sanguinis (2002) 83, 332-338.
Answered by: Technical Support
Date published: 2019-02-22

What is the recommended fixation method for immunofluorescence staining with NuMA (F-11): sc-365532 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-09-29
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Rated 5 out of 5 by from the antibody worked well at 1/500 the antibody worked well at 1/500 by IF and PFA fixed cells.
Date published: 2017-11-13
Rated 5 out of 5 by from Great staining Requested a trial of this antibody for immunofluorescent staining purposes. Wanted to detect mitotic vs. interphase cells and be able to determine spindle orientation. Works fabulously.
Date published: 2017-10-27
Rated 3 out of 5 by from Numa antibody This antibody gave a clean but faint signal by western-blot on 60µg of 293T cells when incubated O/N at 4}© at 1/250 dilution in TBST + 10%SVF. Seems specific but not very strong.
Date published: 2017-09-27
Rated 4 out of 5 by from Works for IF I used this for IF staining of brain sections and it works well
Date published: 2017-08-15
Rated 5 out of 5 by from Produced excellent immunofluorescence nuclear Produced excellent immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-04
Rated 5 out of 5 by from Great Western Blot data of NuMA expression in MOLT Great Western Blot data of NuMA expression in MOLT-4 nuclear extract. -SCBT QC
Date published: 2014-06-23
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