NSE1 Antibody (D-2)recommended for detection of NSE1 of human origin by WB, IP, IF and ELISA

NSE1 Antibody (D-2): sc-376585

NSE1 Antibody (D-2) is rated 5.0 out of 5 by 3.
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Datasheets

    • NSE1 Antibody (D-2) is a mouse monoclonal IgG1 provided at 200 µg/ml
    • raised against amino acids 1-178 mapping at the N-terminus of NSE1 of human origin
    • NSE1 Antibody (D-2) is recommended for detection of NSE1 of human origin by WB, IP, IF and ELISA
    • NSE1 Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NSE1 (D-2): sc-376585.
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I am using NSE1 (D-2): sc-376585 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Trav11
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Great! I tried many of Ab against Nse1 from other company. None of them were able to detect endogenous level of Nse1 in my cell line. I ask for a trial sample and tested it. It works nicely in HepG2 cells.So now I bought a vial from santacruz
Date published: 2018-06-21
Rated 5 out of 5 by from good for WB I applied this antibody sample several days ago.It was diluted to 1:500,primary antibody was incubated overnight at 4℃.Second antibody was diluted to 1:10000 and was incubated at normal temperature 1h. We observed strong and clear band
Date published: 2017-08-15
Rated 5 out of 5 by from Produced positive Western blot data of NSE1 Produced positive Western blot data of NSE1 expression in Hep G2 and PC-3 nuclear extracts. -SCBT QC
Date published: 2013-09-29
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