Nrf2 CRISPR Plasmids (h)human Nrf2-specific CRISPR/Cas9 KO Plasmid, HDR Plasmid, Double Nickase Plasmids, CRISPR Activation Plasmids and Lentiviral Activation Particles

Nrf2 CRISPR Plasmids (h)

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Datasheets

    • Target species: human; for corresponding mouse product, see Nrf2 CRISPR Plasmids (m)
    • CRISPR/Cas9 KO Plasmids consists of Nrf2-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
    • For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
    • Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
    • Gene knockdown or activation can be assayed using Nrf2 Antibody (H-6): sc-518033
    • All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells
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Gene Info

SpeciesGene NameGene IDChromosome LocationIsoform (mRNA) Accession #Protein Accession #OMIM™ Number
HumanNFE2L247802q31.2NM_001145412, NM_001145413, NM_001313900, NM_001313901, NM_001313902, NM_001313903, NM_001313904, NM_006164Q16236600492

Can cells transfected with this plasmid be sorted with flow cytometry? If so, what excitation and emission wavelengths are to be used for the RFP protein?

Asked by: ke265
Thank you for your question. The RFP in our HDR Plasmids is a red (orange) fluorescent protein. The excitation/emission maxima are 553 and 574 nm, respectively. RFP fluorescence in transfected cells can be used to sort positive cells using a flow cytometer with that capability.
Answered by: Tech Support Europe
Date published: 2019-09-24

Last December I purchased CRISPR/Cas9 KO(sc-400017 )plasmids, in April this year I purchased the sc-400017-HDR plasmids for co-transfection, right now I have repeated for several times but did not get the KO cells. SO I ask for your help.

Asked by: chenni
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting these products.
Answered by: Technical Support
Date published: 2019-07-16
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