Nrf2 Antibody (A-10)recommended for detection of ~60 kDa forms of Nrf2 of human origin by WB, IP, IF and ELISA

Nrf2 Antibody (A-10): sc-365949

Nrf2 Antibody (A-10) is rated 4.2 out of 5 by 52.
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Datasheets

    • Nrf2 Antibody (A-10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 37-336 of Nrf2 of human origin
    • Nrf2 Antibody (A-10) is recommended for detection of ~60 kDa forms of Nrf2 of human origin by WB, IP, IF and ELISA
    • Nrf2 Antibody (A-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-365949 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Nrf2 (A-10): sc-365949.
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Does this antibody recognize Nrf2 of mouse or rat origin?

Asked by: laurocarp
Thank you for your question. This antibody has not been tested for mouse and rat use, and can therefore not be warrantied for this use. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-08-27

hello,i want use mouse or rat for WB、Flow cytometry and PCR , can i use the Nrf2 Antibody (A-10): sc-365949 ?

Asked by: Grace 12
Thank you for your question. This antibody is not currently recommended for detection of Nrf2 of mouse or rat origin. It is currently only recommended for human. Please check back for updates!
Answered by: Technical Support
Date published: 2019-08-16

I don't understand what's the difference between anti nrf2 (A10) and anti nrf2 (H10). These antibodies have same datasheet. How is it possible? I've been tested both these antibodies and clearly they are different.

Asked by: Denguezika
Thank you for your question. Both Nrf2 Antibody (H-10) and Nrf2 Antibody (A-10): sc-365949 are mouse monoclonal IgG1 (kappa light chain) antibodies that were raised against amino acids 37-336 of Nrf2 of human origin. Due to the nature of antibody production these two antibodies bind to different locations in the same epitope region of the Nrf2 protein. This is the reason why their properties such as reactivity may differ despite having been produced using the same peptide.
Answered by: Technical Support Europe
Date published: 2019-04-25

Hello, does this Nrf-2 Ab (A-10) work for Immunohistochemistry as the previous one did (sc-13032)? thank you for your help!

Asked by: PabloMR
Nrf-2 Ab (A-10) is recommended for detection of ~60 kDa forms of Nrf2 of human origin by WB, IP, IF and ELISA. If you have any further questions, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 2
Date published: 2019-01-21

Has anybody used this antibody (A-10) or another anti-Nrf2 specific antibody for intracellular/nuclear staining in flow cytometry?

Asked by: AndreasL
Thank you for your question. We have not yet tested any of our Nrf2 antibodies, including Nrf2 (A-10): sc-365949, for flow cytometry, and we are not aware of any of the publications using this antibody using it for this application. Most of the publications are citing this antibody for Western blot, IF and IHC.
Answered by: Technical Support
Date published: 2019-01-21

Hi I wonder about MW. of Nrf2 band. In several Nrf2 antibody companies have shown Nrf2 at above 95-110K. For your antibody why is very close to the actuall Nrf2. I use H-300 two year ago is shown above 90 but now A-10 is close to 50K. do both is correct?

Asked by: narak
Thank you for your question. The molecular weight of Nrf2 has a predicted molecular weight of 65 kDa. According to primary literature, Nrf2 has been detected at 95-110 kDa. It has been suggested that the higher molecular weight may be due to the abundance of acid residues and/or poly-ubiquination of Nrf2. The variation of the higher molecular weights may depend on the composition of SDS-PAGE used. Since there is much discussion on this topic, both predicted and higher molecular weights should be considered. reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3503463/
Answered by: Santa Cruz TS
Date published: 2019-01-21

Hello, I am looking for a Nrf2 antibody for Rats. The ones that were discontinued were for humans, mice, and rats. However, this one only states that it is for humans. Would this not work for rats? If not, what should I use instead?

Asked by: MInhi kang
Thank you for your question. We have not yet tested this monoclonal, Nrf2 Antibody (A-10): sc-365949, for reactivity with rat, so this species is not recommended at this time. The antibody is raised against amino acids 37-336 of Nrf2 of human origin, and this immunogen sequence does share significant (76%) sequence identity with the rat Nrf2 protein sequence. There is a good chance that the antibody will also react with rat, but we still need to confirm this experimentally. We are currently offering a free 10µg sample size of this antibody. Please contact our Technical Service Department if you would like to request a sample to test in rat. Please call us toll-free at (800) 457-3801 option 2, email scbt@scbt.com, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-03-08

Will the Nrf2 Antibody (A-10): sc-365949 work for ChIP applications?

Asked by: Trav11
Yes, we do warranty the Nrf2 Antibody (A-10): sc-365949 for ChIP applications, but we recommend using the much more concentrated "X" version of the antibody, sc-365949 X. We also provide our in-house protocol for Chromatin Immunoprecipitation on our website, for which I have provided the link below: https://www.scbt.com/scbt/resources/protocols/chromatin-immunoprecipitation-assays
Answered by: Tech Service 8
Date published: 2016-12-22
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Rated 5 out of 5 by from 组织和细胞的目的条带大小不一致 细胞和组织Western条带大小不一致。细胞western大小在70KD,而组织在50KD。不知道为什么。但目的条带比较清晰,杂带很少
Date published: 2019-10-16
Rated 5 out of 5 by from good antibody the function of the antibody is good, the picture of IHC is very clear.
Date published: 2019-08-15
Rated 5 out of 5 by from wonderful antibody It is very happy for me to buy this antibody which is efficient.
Date published: 2019-08-13
Rated 3 out of 5 by from Banda debole al peso giusto Ho usato questo Ab in WB 1:500 O/N su 40 ug di lisati cellulari di Hela trattate con H2O2. Il segnale in ECL è molto debole ma corrisponde al peso atteso.
Date published: 2019-05-06
Rated 5 out of 5 by from Nrf2 Antibody A single specific band (60 kDa) was observed in HaCaT. This antibody is good for WB.
Date published: 2019-03-18
Rated 5 out of 5 by from Very good antibody We bought and used this antibody in WB on total lysates from EA.hy926 endothelial cells
Date published: 2019-01-31
Rated 4 out of 5 by from WB Western blotting in C2C12 cells, nice band with low background
Date published: 2019-01-25
Rated 5 out of 5 by from Good for WB A single specific band (60 kDa) was observed in hDPCs. This antibody is good for WB.
Date published: 2018-12-28
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