Nrf2 Antibody (437C2a)recommended for detection of Nrf2 of human origin by WB and IP

Nrf2 Antibody (437C2a): sc-81342

Nrf2 Antibody (437C2a) is rated 4.0 out of 5 by 8.
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Datasheets

    • Nrf2 Antibody (437C2a) is a mouse monoclonal IgG1 (kappa light chain) provided at 100 µg/ml
    • raised against a recombinant protein corresponding to an internal region of Nrf2 of human origin
    • recommended for detection of Nrf2 of human origin by WB and IP
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Nrf2 (A-10): sc-365949 for Nrf2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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At which band is Nrf2 supposed to appear? is it 40KD (according to the figure) or 61KD (according to the molecular weight)??

Asked by: Khal24
Thank you for your question. The Western blot data published at 40 kDa with this antibody is of a human recombinant Nrf2 fusion protein and is not indicative of the endogenous molecular weight of Nrf2. Please see a useful reference for the molecular weight of Nrf2 below. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3503463/
Answered by: Technical Support
Date published: 2019-02-10

I am using Nrf2 (437C2a): sc-81342 antibody for immunoprecipitation, how much antibody should I use?

Asked by: TinTin
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 2 out of 5 by from Confusing Its not showing the band at the correct molecular weight
Date published: 2018-03-07
Rated 2 out of 5 by from Bandas inespecíficas Probé el anticuerpo Nrf2 437C2a en un WB con la línea celular HEPG2,al mismo tiempo que el anticuerpo Nrf2 (A-10): sc-365949. Con el 437C2a aparecen muchas bandas inespecíficas, es mejor el A-10
Date published: 2018-02-22
Rated 5 out of 5 by from Beautiful strong single band Our laboratory got this in replacement of the old ab we had. Glad we got this, did it once and didn't have to repeat the experiment.
Date published: 2017-11-12
Rated 5 out of 5 by from Reliable result Reliable result by western blot detect Nrf2 ,I suggest dilution 1:800,strong band wasdeteced at 40kda, I suggest use positive control for detection.
Date published: 2017-09-09
Rated 5 out of 5 by from very good antibody for human samples This is a very good antibody for human samples, and we will use it for human applications.
Date published: 2017-02-12
Rated 5 out of 5 by from 1:1000 for best results For best results dilute primary antibody 1:1000 and secondary antibody 1:5000 and incubate for just 1 hour with each.
Date published: 2017-02-12
Rated 5 out of 5 by from Great This production is very great with high quanlity and high specificity.
Date published: 2016-11-19
Rated 3 out of 5 by from Strong band in WB with human recombinant Strong band in WB with human recombinant Nrf2 fusion protein. -SCBT QC
Date published: 2014-11-02
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