Nrf1 Antibody (H-4)recommended for detection of Nrf1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Nrf1 Antibody (H-4): sc-28379

Nrf1 Antibody (H-4) is rated 3.8 out of 5 by 12.
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Datasheets

    • Nrf1 Antibody (H-4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 191-475 of Nrf1 of human origin
    • recommended for detection of Nrf1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-28379 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Nrf1 (H-4): sc-28379.
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As mentioned in one of the reviews below, Nfe2l1 runs as bands ~80-120 kD. Has the specificity of this antibody been validated in any way?

Asked by: Benny
Thank you for your question. This mouse monoclonal, Nrf1 Antibody (H-4): sc-28379, is raised against amino acids 191-475 of Nrf1 of human origin and has an epitope that can recognize a smaller fragment of Nrf1, also described as the bZIP region ~25-35 kDa. (Please refer to PMID's: 9826775 and 11867748 for more information about this lower molecular weight fragment. Also, a product citation using this antibody, PMID: 22216197). There are currently two full-sized (23-132 kDa) Western blot images shown for this antibody on our website where both the glycosylated full-length Nrf1, and the lower molecular weight bZIP region are observed in various cell lines, including: HeLa, HepG2 and NIH/3T3 whole cell lysates.
Answered by: Technical Support
Date published: 2017-03-01

When should the TransCruz reagent sc-28379 X be used instead of the standard form sc-28379?

Asked by: AbPolly
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from It is good for WB for mouse hippocampus It is good for WB. For mouse hippocampus in specific treatment you can get bands for whole length and bZIP region
Date published: 2018-12-12
Rated 5 out of 5 by from Quite good antibody 1:100 diluted for wb of rat heart protein,the band is clear and special
Date published: 2017-12-31
Rated 5 out of 5 by from Antibody for WB The antibody is uesed to make western blotting,with clean background,and clear stripe.
Date published: 2017-09-11
Rated 5 out of 5 by from 效果挺好的!! 效果一般,只能用一次不能回收再使用,条带有些杂带,背景比较脏。洗不干净的。抗体的效价不高的,不知道是不是表达量本身就低还是抗体的活性低呢
Date published: 2017-08-20
Rated 1 out of 5 by from questionable results This antibody detects several bands: one around 26 kDa, one around 45 kDa, and a double band around 95 kDa. None of these bands can be Nrf1 (nrf1 should be detcted around 70 kDa).
Date published: 2017-08-03
Rated 4 out of 5 by from Good antibody for WB Worked very well in WB at 1:500 dilution with mouse brain tissue lysate.
Date published: 2017-07-25
Rated 1 out of 5 by from Detects a non-specific band First, as many may have noticed, molecular weight of the protein detected by this antibody (<35 kDA) differs from that predicted (56 kDa). We established a Tet-On system with a myc-tagged human NRF1. Upon induction, myc-tag antibody detects massive overexpression of the protein with predicted molecular weight. No increase in expression is detected in these same samples by H4 antibody, which detects what appears to be nonspecific bands at ~40, ~60 and ~80 kDa
Date published: 2017-04-10
Rated 5 out of 5 by from Reliable Antibody, Great for WB This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-18
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