Nrf1 Antibody (G-5)recommended for detection of Nrf1 of mouse, rat and human origin by WB, IP, IF and ELISA

Nrf1 Antibody (G-5): sc-515360

Nrf1 Antibody (G-5) is rated 4.3 out of 5 by 3.
  • y_2019, m_12, d_7, h_22
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_515360, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 33ms
  • REVIEWS, PRODUCT
5
1
4
2
3
0
2
0
1
0

Datasheets

    • Nrf1 Antibody (G-5) is a mouse monoclonal IgG2b provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 747-772 at the C-terminus of Nrf1 of human origin
    • Nrf1 Antibody (G-5) is recommended for detection of Nrf1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Nrf1 Antibody (G-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-515360 X, 200 µg/0.1 ml)
    • blocking peptide, sc-515360 P
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Nrf1 (G-5): sc-515360.
Submit a review for this product and receive 15 CruzCredits

Is the blocking peptide sc-515360P equivalent to the peptide against which the antibody was raised or is it a truncation thereof? If the latter is the case, which sequence does peptide sc-515360P have?

Asked by: Questionasker01
Thank you for your question. The sc-516360P are peptides comprised the sequences used to generate the antibody and are incubated with the antibody in great excess. The exact sequence is considered to be proprietary information. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: SCBT Heidelberg Support
Date published: 2018-12-22

What application is the blocking peptide sc-515360 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-03-01
  • y_2019, m_12, d_7, h_22CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_515360, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 4ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from 良く染まります! IFに使いました。核内移行についての評価をしていたのですが、 期待以上に良く染まり評価がしやすかったです。今後も継続して 使用させて頂きたいと思います。
Date published: 2018-02-08
Rated 4 out of 5 by from works well for western blotting recognized a single band of the right size on western blotting of mouse whole cell extract at 1:1000 dilution with 3% BSA in PBST.
Date published: 2017-12-06
Rated 4 out of 5 by from great Antibody Amount used WB was 1:500 in BSA and 1:7500 of secondary antibody on mitochondrial extracts
Date published: 2017-08-16
  • y_2019, m_12, d_7, h_22
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.3
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_515360, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 4ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.