Anti-NR2E1 Antibody (B-10) recommended for detection of NR2E1 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-NR2E1 Antibody (B-10): sc-377240

Anti-NR2E1 Antibody (B-10) is rated 4.0 out of 5 by 4.
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Datasheets

    • Anti-NR2E1 Antibody (B-10) is a mouse monoclonal IgG1 κ NR2E1 antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 89-190 mapping within an internal region of NR2E1 of human origin
    • Anti-NR2E1 Antibody (B-10) is recommended for detection of NR2E1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine and porcine
    • Anti-NR2E1 Antibody (B-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • TransCruz reagent for ChIP application (sc-377240 X, 200 µg/0.1 ml)
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NR2E1 (B-10): sc-377240.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for NR2E1 Antibody (B-10) for WB applications. These reagents are now offered in bundles with NR2E1 Antibody (B-10) (see ordering information below).
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Are there any issues with using sc-377240: NR2E1 (B-10) mouse monoclonal primary antibody on mouse samples?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-377240 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
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Rated 5 out of 5 by from 美しいバンドが検出されます。 WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 1 out of 5 by from Did not work on mouse brain tissue (Thalamus) Immunofluorescence (1:200) did not work on frozen section from 4% PFA fixed embryonic mouse brain tissue. 3-5 min retrieval with citric buffer (PH6.0).
Date published: 2017-10-11
Rated 5 out of 5 by from Good for WB The NR2E1 Antibody (B-10): sc-377240 works well for Western blot applications, showing strong band with minimal background noise.
Date published: 2017-02-02
Rated 5 out of 5 by from Produced positive Western blot data of NR2E1 Produced positive Western blot data of NR2E1 expression in Jurkat and SH-SY5Y whole cell lysates. -SCBT QC
Date published: 2014-03-27
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