NPT1 Antibody (4B4D1)recommended for detection of NPT1 of human origin by WB and ELISA

NPT1 Antibody (4B4D1): sc-517230

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Datasheets

    • NPT1 Antibody (4B4D1) is a mouse monoclonal IgG2a provided at 100 µg/ml
    • raised against toxin binding protein fragment of NPT1 of human origin
    • recommended for detection of NPT1 of human origin by WB and ELISA
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How much sample do you recommend loading on the gel for Western blot detection using NPT1 (4B4D1): sc-517230?

Asked by: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-27
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