Anti-NPC1 Antibody (G-1) recommended for detection of NPC1 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-NPC1 Antibody (G-1): sc-271335

Anti-NPC1 Antibody (G-1) is rated 3.7 out of 5 by 3.
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Datasheets

    • Anti-NPC1 Antibody (G-1) is a mouse monoclonal IgA (kappa light chain) NPC1 antibody provided at 200 µg/ml
    • raised against amino acids 516-630 of NPC1 of human origin
    • recommended for detection of NPC1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of NPC1 (G-1): sc-271335.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for NPC1 Antibody (G-1). This work is in progress.
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Hi, is NPC1 (G-1) have been checked in Huh7 cells and CHO-K1 cells?

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us directly at 021-60936350, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service SH
Date published: 2022-01-18

Has this antibody been validated?

Asked by: Lucy10
Thank you for your question. This antibody has been validated in Western blotting (WB) and immunofluorescent (IF) staining research applications. Our website is updated with the most current data available for our products. Please contact our Technical Service Department for further support.
Answered by: Technical Support
Date published: 2019-06-21

What is the recommended fixation method for immunofluorescence staining with NPC1 (G-1): sc-271335 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from No signal in WB No detectable signals in several trials on different ovarian cancer cell extracts (dilution 1:500, O.N. incubation)
Date published: 2021-02-10
Rated 5 out of 5 by from Superb immunofluorescence cytoplasmic staining Superb immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-12
Rated 5 out of 5 by from Satisfactory Western Blot data of NPC1 expression Satisfactory Western Blot data of NPC1 expression in ES-2 whole cell lysate. -SCBT QC
Date published: 2014-02-09
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