Anti-NPC1 Antibody (E-9) recommended for detection of NPC1 of human origin by WB, IP, IF and ELISA

Anti-NPC1 Antibody (E-9): sc-271334

Anti-NPC1 Antibody (E-9) is rated 5.0 out of 5 by 1.
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Datasheets

    • NPC1 Antibody (E-9) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 516-630 of NPC1 of human origin
    • recommended for detection of NPC1 of human origin by WB, IP, IF and ELISA
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for NPC1 Antibody (E-9) for WB applications. These reagents are now offered in bundles with NPC1 Antibody (E-9) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-271334: NPC1 (E-9) monoclonal antibody?

Asked by: AbPolly
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Produced nice Western blot data of NPC1 expression Produced nice Western blot data of NPC1 expression in ES-2 whole cell lysate. -SCBT QC
Date published: 2014-11-28
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